• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>World Journal of Gastroenterology >Antineoplastic effects of octreotide on human gallbladder cancer cells in vitro
【24h】

Antineoplastic effects of octreotide on human gallbladder cancer cells in vitro

机译：奥曲肽对人胆囊癌细胞的体外抗肿瘤作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AIM: To investigate whether octreotide can inhibit the growth of human gallbladder cancer cells in vitro and to elucidate the antineoplastic mechanism of octreotide in gallbladder cancer. METHODS: A human gallbladder cancer cell line, GBC-SD, was cultured in vitro. The antiproliferative effects of octreotide were examined by means of an MTT assay and a colony forming ability assay. Morphological variation was investigated under scanning electron microscopy and transmission electron microscopy. Cell cycle analysis and apoptosis rate was evaluated by flow cytometry (FCM) after staining by propidium iodide. DNA fragmentation was assayed by agarose gel electrophoresis. Immunohistochemical staining was performed to evaluate the expressions of mutant-type p53 and bcl-2. RESULTS: The growth curve and colony forming ability assay showed significant inhibition of octreotide to the proliferation of GBC-SD cells in culture in a time- and dose-dependent manner. After exposure to octreotide, GBC-SD cells showed typically apoptotic characteristics, including morphological changes of chromatin condensation, vacuolar degeneration, nucleus fragmentation and apoptotic body formation. In FCM profile apoptotic cells showed increased sub-G_1 peaks in the octreotide group, significantly higher than the control group (P=0.013). There was also an augmentation in the cell proportion of G_0/G_1 phase (P=0.015), while the proportion of S phase and G_2/M phase remained unchanged (P=0.057 and P=0.280, respectively). DNA agarose gel electrophoresis displayed a ladder after exposure to 1 000 nmol/L octreotide. After being treated with octreotide, the expressions of both mutant-type p53 and bcl-2 decreased considering the percentage of positive cells (P<0.05). CONCLUSION: Octreotide has a negative action to the proliferation of GBC-SD cells, and the mechanism may be related to cytostatic and cytotoxic effects. The reduction of mutant-type p53 and bcl-2 expressions may be associated with the apoptosis induced by octreotide.
机译：目的：探讨奥曲肽在体外是否能抑制人胆囊癌细胞的生长，并阐明奥曲肽在胆囊癌中的抗肿瘤作用机制。方法：体外培养人胆囊癌细胞系GBC-SD。通过MTT测定和菌落形成能力测定来检查奥曲肽的抗增殖作用。在扫描电子显微镜和透射电子显微镜下研究形态变化。碘化丙锭染色后，通过流式细胞术（FCM）评估细胞周期分析和凋亡率。通过琼脂糖凝胶电泳测定DNA片段化。进行免疫组织化学染色以评估突变型p53和bcl-2的表达。结果：生长曲线和集落形成能力测定显示奥曲肽以时间和剂量依赖性方式显着抑制培养物中GBC-SD细胞的增殖。暴露于奥曲肽后，GBC-SD细胞通常表现出凋亡特征，包括染色质浓缩，液泡变性，细胞核碎裂和凋亡小体形成的形态变化。在FCM中，凋亡细胞在奥曲肽组中显示出亚G_1峰的增加，明显高于对照组（P = 0.013）。 G_0 / G_1相的细胞比例也有增加（P = 0.015），而S相和G_2 / M相的比例则保持不变（分别为P = 0.057和P = 0.280）。暴露于1000 nmol / L奥曲肽后，DNA琼脂糖凝胶电泳显示出阶梯。用奥曲肽治疗后，考虑到阳性细胞的百分比，突变型p53和bcl-2的表达均降低（P <0.05）。结论：奥曲肽对GBC-SD细胞的增殖具有负作用，其机制可能与细胞抑制作用和细胞毒作用有关。突变型p53和bcl-2表达的减少可能与奥曲肽诱导的细胞凋亡有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号