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首页> 外文期刊>Bulletin of the National Research Centre >Intraspecific, Interspecific and Intergeneric Protoplast Fusion for the Improvement of Cellulases Production in Fungi
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Intraspecific, Interspecific and Intergeneric Protoplast Fusion for the Improvement of Cellulases Production in Fungi

机译：种内，种间和属间原生质体融合改善真菌中纤维素酶生产。

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This investigation aimed to improve cellulase enzymes productivity in Trichoderma reesei upon protoplast fusion technique in three different combinations; the first one is an intraspecific fusion between the two induced mutants S/26 and 5/32 which were selected previously by the authres as highly cellulases producers, from the original T. reesei strain NRRL. 12368. The second is an interspecific fusion between T. reesei (S/26) and T. harzianum, while the third one is an intergeneric fusion between T. reesei (S/26) and Aspergillus niger. The first fusion induced 11 fusants, one of which (F1/10) showed the highest cellulases activity producing 21.4%, 13.3% and 13.3% degradation of filter paper enzyme (FPase), carboxyl methyl cellulase (CMCase) and 3-glucosidase, respectively, more than the higher parent (S/26). The second fusion induced 8 fusants, one of which (F2/8) produced 7.1%, 6.7% and 13.3% FPase, CMCase and β-glucosidase, more than the higher parent (S/26), respectively. The third fusion induced 15 fusants, all of which were lower than T. reesei (S/26) in cellulases activity except one fusant (F4/6) which showed 7.14% increase of FPase and 2.67% of β-glucosidase more than its higher parent (S/26), while its CMCase activity was the same as that of this parent.
机译：这项研究旨在通过三种不同组合的原生质体融合技术提高里氏木霉的纤维素酶生产率。第一个是两个诱导的突变体S / 26和5/32之间的种内融合，突变体S / 26和5/32以前是被原先从里氏木霉NRRL菌株选为高纤维素酶生产者的。 12368.第二个是里氏木霉（S / 26）和哈茨木霉（T. harzianum）之间的种间融合，而第三个是里氏木霉（S / 26）和黑曲霉（Aspergillus niger）之间的属间融合。第一次融合诱导的11种融合剂，其中一种（F1 / 10）表现出最高的纤维素酶活性，分别导致滤纸酶（FPase），羧甲基纤维素酶（CMCase）和3-葡糖苷酶降解21.4％，13.3％和13.3％。 ，比上级父母（S / 26）还要多。第二次融合诱导了8种融合蛋白，其中一种（F2 / 8）产生的FPase，CMCase和β-葡萄糖苷酶分别比高亲本（S / 26）多7.1％，6.7％和13.3％。第三次融合诱导了15种融合蛋白，所有纤维素酶的活性均低于里氏木霉（S / 26），只有一种融合蛋白（F4 / 6）的FPase增加了7.14％，β-葡萄糖苷酶增加了2.67％。亲本（S / 26），而其CMCase活性与此亲本相同。

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