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首页> 外文期刊>Cell Research >Derivation of human embryonic stem cell lines from parthenogenetic blastocysts
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Derivation of human embryonic stem cell lines from parthenogenetic blastocysts

机译：单性生殖胚泡衍生人胚胎干细胞系

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Parthenogenesis is one of the main, and most useful, methods to derive embryonic stem cells (ESCs), which may be an important source of histocompatible cells and tissues for cell therapy. Here we describe the derivation and characterization of two ESC lines (hPES-1 and hPES-2) from in vitro developed blastocysts following parthenogenetic activation of human oocytes. Typical ESC morphology was seen, and the expression of ESC markers was as expected for alkaline phosphatase, octamer-binding transcription factor 4, stage-specific embryonic antigen 3, stage-specific embryonic antigen A, TRA-1-60, and TRA-1-81, and there was absence of expression of negative markers such as stage-specific embryonic antigen 1. Expression of genes specific for different embryonic germ layers was detected from the embryoid bodies (EBs) of both hESC lines, suggesting their differentiation potential in vitro. However, in vivo, only hPES-1 formed teratoma consisting of all three embryonic germ layers (hPES-2 did not). Interestingly, after continuous proliferation for more than 100 passages, hPES-1 cells still maintained a normal 46 XX karyotype; hPES-2 displayed abnormalities such as chromosome translocation after long term passages. Short Tandem Repeat (STR) results demonstrated that the hPES lines were genetic matches with the egg donors, and gene imprinting data confirmed the parthenogenetic origin of these ES cells. Genome-wide SNP analysis showed a pattern typical of parthenogenesis. All of these results demonstrated the feasibility to isolate and establish human parthenogenetic ESC lines, which provides an important tool for studying epigenetic effects in ESCs as well as for future therapeutic interventions in a clinical setting.
机译：单性生殖是获得胚胎干细胞（ESC）的主要方法之一，也是胚胎干细胞和组织治疗细胞的重要来源。在这里，我们描述了人类卵母细胞孤雌生殖激活后体外发育的胚泡中的两个ESC系（hPES-1和hPES-2）的衍生和特征。观察到典型的ESC形态，并且ESC标记的表达与碱性磷酸酶，八聚体结合转录因子4，阶段特异性胚胎抗原3，阶段特异性胚胎抗原A，TRA-1-60和TRA-1预期的一样。 -81，并且不存在阴性标记物的表达，例如阶段特异性胚胎抗原1。从两个hESC系的胚状体（EB）中检测到了针对不同胚芽层的特异性基因的表达，表明它们在体外具有分化潜能。 。但是，在体内，只有hPES-1形成了由所有三个胚芽层组成的畸胎瘤（hPES-2没有）。有趣的是，在连续增殖超过100代之后，hPES-1细胞仍保持正常的46 XX核型。长期传代后，hPES-2显示出异常，例如染色体易位。短串联重复序列（STR）结果表明，hPES系与卵子供体是遗传匹配的，并且基因印迹数据证实了这些ES细胞的孤雌生殖起源。全基因组SNP分析显示了孤雌生殖的典型模式。所有这些结果证明了分离和建立人类孤雌生殖ESC系的可行性，这为研究ESC中的表观遗传效应以及在临床环境中进行未来的治疗性干预提供了重要的工具。

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