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首页> 外文期刊>Cell Research >Molecular and functional characterization of sulfiredoxin homologs from higher plants.
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Molecular and functional characterization of sulfiredoxin homologs from higher plants.

机译：来自高等植物的硫吡菌毒素同系物的分子和功能表征。

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By reducing cysteine-sulfinic acid in oxidized peroxiredoxin, sulfiredoxin (Srx) plays an important role in oxidation stress resistance in yeast and human cells. Here, we report the first molecular and functional characterization of Srx homolog from higher plants. Bioinformatic analysis revealed the presence of potential Srx encoding sequences in both monocot and dicot plant species. Putative plant Srx proteins exhibited significant identities to their orthologs from yeast and human, and contained the conserved signature sequence and residues essential for catalysis. However, unlike yeast and human orthologs, plant Srxs were all predicted to possess chloroplast transit peptide in their primary structure. The Srx proteins from Arabidopsis and rice (designated as AtSrx and OsSrx, respectively) complemented functional deficiency of Srx in the SRX1 deletion yeast cells. A GFP fusion protein of AtSrx was targeted to chloroplast in Arabidopsis mesophyll protoplast. AtSrx transcription occurred in both vegetative and reproductive organs, and the highest transcript level was detected in leaves. Under oxidation stress, AtSrx transcript level was substantially increased, which paralleled with enhanced transcription of 2-Cys peroxiredoxins that have been found essential in maintaining chloroplast redox balance. In addition to oxidation stress, osmotic/water deficit or cold treatments also raised AtSrx transcript level. Consistent with above findings, the knock-out mutant of AtSrx was significantly more susceptible to oxidation stress than wild type Arabidopsis plant. Taken together, the results of this work indicate the existence of functional Srx homolog in higher plants that is essential for plants to cope with oxidation stress.Cell Research (2006) 16: 287-296. doi:10.1038/sj.cr.7310036; published online 16 March 2006.
机译：通过还原氧化的过氧化物酶中的半胱氨酸亚磺酸，磺基毒素（Srx）在酵母和人类细胞的抗氧化应激中起重要作用。在这里，我们报告来自高等植物的Srx同源物的第一个分子和功能表征。生物信息学分析表明，在单子叶植物和双子叶植物中都存在潜在的Srx编码序列。推定的植物Srx蛋白与其酵母和人的直系同源物具有重要的同一性，并且包含保守的签名序列和催化必不可少的残基。然而，与酵母和人类直系同源物不同，植物Srxs均被预测在其一级结构中具有叶绿体转运肽。来自拟南芥和水稻的Srx蛋白（分别命名为AtSrx和OsSrx）弥补了SRX1缺失酵母细胞中Srx的功能缺陷。 AtSrx的GFP融合蛋白靶向拟南芥叶肉原生质体中的叶绿体。营养和生殖器官均发生AtSrx转录，并且在叶片中检测到最高的转录水平。在氧化胁迫下，AtSrx转录水平显着提高，这与2-Cys过氧化物酶的转录增强同时被发现对于维持叶绿体氧化还原平衡至关重要。除氧化应激外，渗透/缺水或冷处理还提高了AtSrx转录水平。与上述发现一致，AtSrx的敲除突变体比野生型拟南芥植物更容易受到氧化胁迫的影响。综上所述，这项工作的结果表明高等植物中存在功能性Srx同源物，这对于植物应对氧化胁迫至关重要。CellResearch（2006）16：287-296。 doi：10.1038 / sj.cr.7310036;在线发布于2006年3月16日。

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