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Identification of ABA-responsive genes in rice shoots via cDNA macroarray

机译：通过cDNA芯片鉴定水稻芽中的ABA反应基因

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摘要

Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and 12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least one time, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealed that the putative encoded proteins were involved in different possible processes, including transcription, metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones were found to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signal transduction or metabolism was confirmed by reverse transcription PCR. In addition, some clones were further shown to be regulated by other plant growth regulators including auxin and brassinosteroid, which, however, indicated the complicated interactions of plant hormones. Possible signal transduction pathways involved in ABA were discussed.
机译：植物激素脱落酸（ABA）对于许多植物的生长和发育过程至关重要，包括种子成熟，发芽和对环境因素的响应。为了检测可能的ABA相关信号转导途径，我们试图通过cDNA宏阵列技术以ABA处理的水稻幼苗为材料（分别处理2、4、8和12 h）分离ABA调控的基因。在测试的6144个cDNA克隆中，分离出至少诱导一次抑制或抑制一次的37个差异克隆。其中30和7分别被上调或下调。序列分析表明，推定的编码蛋白参与了不同的可能过程，包括转录，代谢和抗性，光合作用，信号转导和种子成熟。发现6个cDNA克隆编码功能未知的蛋白质。通过逆转录PCR证实了ABA对7种与信号转导或代谢有关的克隆的调控。此外，一些克隆进一步显示受其他植物生长调节剂（包括生长素和油菜素甾体）的调节，但是，这表明植物激素之间的相互作用复杂。讨论了可能与ABA有关的信号转导途径。

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