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首页> 外文期刊>Cell and Tissue Research >Modulation of cell proliferation during palatogenesis by the interplay between Tbx3 and Bmp4
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Modulation of cell proliferation during palatogenesis by the interplay between Tbx3 and Bmp4

机译：Tbx3和Bmp4之间的相互作用调节ulation形成过程中的细胞增殖

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摘要

During secondary palate development, two shelves are elevated to a horizontal position above the tongue through a process involving many cellular mechanisms, including proliferation. In particular, the expression patterns of Tbx3 and Bmp4, which are colocalized at embryonic day 13.5 (E13.5) and have unique expression patterns in specific regions at E14.5, have been investigated in early mouse palatogenesis. Tbx3 expression is reported to be associated with Bmp4 signaling during the process of organogenesis in other areas, such as limb development. However, the function of Tbx3 and the relationship between Tbx3 and Bmp4 in palate development have not been determined. We have examined the gene expression pattern and cell proliferation in order to understand the mutual interactions and function of Tbx3 and Bmp4. An electroporation method was used to investigate the altered pattern of these genes after their over-expression in organ cultures. NOGGIN protein-soaked beads were also implanted into the cultured palate to determine the function of Bmp4 in palatogenesis. After electroporation and NOGGIN bead implantation, the number of PCNA-positive cells was counted. The results showed that Tbx3 and Bmp4 strongly up- and down-regulated each other in order to control the proliferation of the palatal shelf. Thus, Tbx3 expression is induced by Bmp4 in the mesenchyme of the anterior palatal shelves, whereas mesenchymal expression of Tbx3 down-regulates Bmp4 expression in the mesenchyme of the palate. The harmonization between Tbx3 and Bmp4 therefore controls cell proliferation to regulate secondary palate development.
机译：在第二secondary发展期间，通过涉及许多细胞机制（包括增殖）的过程，将两个架子升高到舌头上方的水平位置。特别地，已经在早期小鼠pa裂中研究了在胚胎第13.5天（E13.5）共定位并在E14.5的特定区域具有独特表达模式的Tbx3和Bmp4的表达模式。据报道，Tbx3表达与其他区域（例如肢体发育）器官发生过程中的Bmp4信号传导相关。然而，尚未确定Tbx3的功能以及Tbx3和Bmp4在味觉发育中的关系。我们已经检查了基因表达模式和细胞增殖，以了解Tbx3和Bmp4的相互作用和功能。在器官培养物中过表达后，使用电穿孔方法研究了这些基因的改变模式。也将浸有NOGGIN蛋白的珠子植入培养的pa中，以确定Bmp4在pa形成中的功能。电穿孔和NOGGIN珠植入后，计数PCNA阳性细胞的数量。结果表明，Tbx3和Bmp4相互强烈上调和下调，以控制the架的增殖。因此，Tbx3的表达是由Bmp4在前lat架子的间质中诱导的，而Tbx3的间质表达会下调上颚的间质中的Bmp4表达。因此，Tbx3和Bmp4之间的协调控制细胞增殖，从而调节继发pa的发育。

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  • 来源
    《Cell and Tissue Research》 |2007年第2期|285-292|共8页
  • 作者

    Jong-Min Lee; Jae-Young Kim; Kyoung-Won Cho; Min-Jung Lee; Sung-Won Cho; Yanding Zhang; Seong-Kyu Byun; Choong-Kook Yi; Han-Sung Jung;

  • 作者单位

    Division in Anatomy and Developmental Biology Department of Oral Biology College of Dentistry Yonsei University 134 Shinchon-Dong Seodaemoon-Gu Seoul 120-752 South Korea;

    Division in Anatomy and Developmental Biology Department of Oral Biology College of Dentistry Yonsei University 134 Shinchon-Dong Seodaemoon-Gu Seoul 120-752 South Korea;

    Division in Anatomy and Developmental Biology Department of Oral Biology College of Dentistry Yonsei University 134 Shinchon-Dong Seodaemoon-Gu Seoul 120-752 South Korea;

    Division in Anatomy and Developmental Biology Department of Oral Biology College of Dentistry Yonsei University 134 Shinchon-Dong Seodaemoon-Gu Seoul 120-752 South Korea;

    Division in Anatomy and Developmental Biology Department of Oral Biology College of Dentistry Yonsei University 134 Shinchon-Dong Seodaemoon-Gu Seoul 120-752 South Korea;

    College of Bioengineering Fujian Normal University Fuzhou 350007 People’s Republic of China;

    Department of Oral Maxillofacial Surgery Yonsei University Seoul 120-752 South Korea;

    Department of Oral Maxillofacial Surgery Yonsei University Seoul 120-752 South Korea;

    Division in Anatomy and Developmental Biology Department of Oral Biology College of Dentistry Yonsei University 134 Shinchon-Dong Seodaemoon-Gu Seoul 120-752 South Korea;

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  • 正文语种 eng
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  • 关键词

    Palatogenesis; Cell proliferation; Tbx3; Bmp4; Electroporation; NOGGIN; Mouse (ICR);

    机译：癌变;细胞增殖;Tbx3;Bmp4;电化;头蛋白;小鼠（ICR）;

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