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Testing the Effects of Barriers on the Genetic Connectivity in Podocnemis erythrocephala (Red-Headed Amazon River Turtle): Implications for Management and Conservation

机译:测试屏障对Podocnemis erythrocephala(红头亚马逊河龟)的遗传连通性的影响:对管理和保护的意义

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In contrast to the other Podocnemis species, Podocnemis erythrocephala (Pleurodira: Podocnemididae) is restricted to blackwater and clearwater rivers and lakes, including the Negro River basin, and several other tributaries of the eastern Amazon River basin. In order to test the effects of geographic barriers on genetic connectivity of P. erythrocephala, 10 localities sampled throughout the Brazilian Amazon basin were studied. Two hundred and forty-six specimens were sequenced for the mitochondrial control region. Analysis of molecular variance confirmed the existence of population subdivision (Phi(ST) = 0.28, p < 0.001). In pairwise comparisons, the values of the fixation index Phi(ST) were significant in almost all comparisons involving the Sao Gabriel da Cachoeira (Negro River), Jau National Park (Jau River), and Barreirinha (Andira River), indicating that these populations are genetically differentiated. Genetic differentiation is most likely explained by the presence of rapids and waterfalls separating the Sao Gabriel da Cachoeira (Negro River) and Jau National Park (Jau River) populations from the remaining populations. Populations from Barreirinha (Andira River) were also genetically differentiated, providing support for the hypothesis that the Amazon River is a geographic barrier for this species. Although distributed over a large geographic area, the remaining 7 localities were not differentiated from one another (p < 0.005), suggesting that these localities are part of a panmictic population distributed throughout the central Amazon basin. We recommend that the 4 structured populations be treated as separate management units.
机译:与其他Podocnemis物种相反,Podocnemis erythrocephala(Pleurodira:Podocnemididae)仅限于黑水和清水河流和湖泊,包括黑人河流域和东部亚马逊河流域的其他支流。为了测试地理障碍对红斑狼疮遗传连通性的影响,研究了整个巴西亚马逊流域的10个采样点。对246个标本进行线粒体控制区域测序。分子方差分析证实了种群细分的存在(Phi(ST)= 0.28,p <0.001)。在成对比较中,固定指数Phi(ST)的值在涉及Sao Gabriel da Cachoeira(内格罗河),Jau国家公园(Jau河)和Barreirinha(安迪拉河)的几乎所有比较中均很显着,表明这些人群有遗传差异。遗传分化最有可能是由将圣加布里埃尔·达·卡乔埃拉(内格罗河)和茹阿国家公园(茹阿河)种群与其余种群分隔开的急流和瀑布的存在来解释的。来自Barreirinha(安迪拉河)的种群也进行了遗传分化,为以下假设提供了依据:亚马逊河是该物种的地理障碍。尽管分布在一个较大的地理区域中,但其余7个地区没有彼此区分开(p <0.005),这表明这些地区是整个亚马逊河流域中部的恐慌人口的一部分。我们建议将4个结构化人口视为独立的管理单位。

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