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首页> 外文期刊>Chemosphere >Detoxification of hydroxylated polychlorobiphenyls by Sphingomonas sp strain N-9 isolated from forest soil
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Detoxification of hydroxylated polychlorobiphenyls by Sphingomonas sp strain N-9 isolated from forest soil

机译：从森林土壤中分离的鞘氨醇单胞菌菌株N-9对羟基化的多氯联苯进行解毒

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摘要

To examine the biodegradation of hydroxylated polychlorobiphenyls (OH-PCBs), we isolated Sphingomonas sp. strain N-9 from forest soil using mineral salt medium containing 4-hydroxy-3-chlorobiphenyl (4OH-3CB) at the concentration of 10 mg/L. Following incubation with strain N-9, the concentration of 4OH-3CB decreased in inverse proportion to strain N-9 proliferation, and it was converted to 3-chloro-4-hydroxybenzoic acid (4OH-3CBA) after 1 day. We observed that strain N-9 efficiently degraded lowly chlorinated OH-PCBs (1-4 Cl), while highly chlorinated OH-PCBs (5-6 Cl) were less efficiently transformed. Additionally, strain N-9 degraded PCBs and OH-PCBs with similar efficiencies, and the efficiency of OH-PCB degradation was dependent upon the positional relationships between OH-PCB hydroxyl groups and chlorinated rings. OH-PCB biodegradation may result in highly toxic products, therefore, we evaluated the cytotoxicity of two OH-PCBs [4OH-3CB and 4-hydroxy-3,5-dichlorobiphenyl (4OH-3,5CB)] and their metabolites [4OH-3CBA and 3,5-chloro-4-hydroxybenzoic acid (4OH-3,5CBA)] using PC12 rat pheochromocytoma cells. Our results revealed that both OH-PCBs induced cell membrane damage and caused neuron-like elongations in a dose-dependent manner, while similar results were not observed for their metabolites. These results indicated that strain N-9 can convert OH-PCBs into chlorohydroxybenzoic acids having lower toxicity. (C) 2016 Published by Elsevier Ltd.
机译：为了检查羟基化的多氯联苯（OH-PCBs）的生物降解，我们分离了鞘氨醇单胞菌。使用含有4-羟基-3-氯联苯（4OH-3CB）的矿物盐培养基以10 mg / L的浓度从森林土壤中分离N-9菌株。与菌株N-9孵育后，4OH-3CB的浓度与菌株N-9增殖成反比地降低，并在1天后转化为3-氯-4-羟基苯甲酸（4OH-3CBA）。我们观察到，菌株N-9有效降解了低氯OH-PCB（1-4 Cl），而高氯OH-PCB（5-6 Cl）转化效率较低。此外，菌株N-9降解PCB和OH-PCB的效率相似，并且OH-PCB降解的效率取决于OH-PCB羟基与氯化环之间的位置关系。 OH-PCB的生物降解可能会产生高毒性的产品，因此，我们评估了两种OH-PCB [4OH-3CB和4-羟基-3,5-二氯联苯（4OH-3,5CB）]及其代谢物[4OH- 3CBA和3,5-氯-4-羟基苯甲酸（4OH-3,5CBA）]使用PC12大鼠嗜铬细胞瘤细胞。我们的结果表明，两种OH-PCBs均以剂量依赖的方式诱导细胞膜损伤并引起神经元样伸长，而其代谢产物未观察到相似的结果。这些结果表明，菌株N-9可以将OH-PCBs转化为毒性较低的氯羟基苯甲酸。 （C）2016由Elsevier Ltd.出版

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