Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization

QIU Jiwen; JIN Demin; LI Chuanyou

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Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization

机译：通过广泛杂交将Trisapcum dactyloides DNA整合到小麦基因组中的分子验证

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RAPD and RFLP analyses of double haploid lines which derived from hybridization between hexaploid wheat (Triticum aestivum L. 2n=420 and eastern gamagrass (Tripsacum dactyloides L. 2n=4x=27) are reported. Two of the 340 Operon primers have been screened, which stably amplified Tripsacum dactyloices (male parent) specific bands in the double haploid lines. These results confirm the fact that Tripsacum dactyloides DNA has been integrated into wheat genome by sexual hybridization at molecular level.

机译：对六倍体小麦（Triticum aestivum L. 2n = 420和东部gamagrass（Tripsacum dactyloides L. 2n = 4x = 27）之间杂交产生的双单倍体系进行了RAPD和RFLP分析，已筛选出340种Operon引物中的两种，该基因稳定扩增了双单倍体系中的Trisapcum dactyloices（雄性亲本）特异条带，证实了Tapsacum dactyloides DNA已通过分子杂交在小麦基因组中整合。

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《Chinese science bulletin》 |2000年第6期|p.528-531|共4页
作者
QIU Jiwen; JIN Demin; LI Chuanyou;
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正文语种 eng
中图分类自然科学现状及发展;
关键词
bread wheat; Tripsacum dactyloides; wide hybridization;

机译：面包小麦Tripsacum dactyloides;广泛杂交;

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1. Characterization of the complete chloroplast genome of the Eastern gamagrass, Tripsacum dactyloides [J] . Yanfang Wang, Mingzhu Zhao, Li Li, Mitochondrial DNA Part B . 2017,第2期

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2. Integration of maize nuclear and mitochondrial DNA into the wheat genome through somatic hybridization [J] . Xu CH., Xia GM., Zhi DY., Plant Science: An International Journal of Experimental Plant Biology . 2003,第5期

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机译：集成DNA生物芯片制造和杂交自动化的分子动力学
5. Genetic and physical mapping of chromosome 4E of tall wheatgrass, Thinopyrum elongatum, and, Molecular detection of rDNA sequences indicates a novel genome donor in the polyploid genus Thinopyrum. [D] . Arterburn, Matthew Keith. 2006

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6. Integrating genome-wide DNA methylation and mRNA expression profiles identified different molecular features between Kashin-Beck disease and primary osteoarthritis [O] . Yan Wen, Ping Li, Jingcan Hao, 2018

机译：整合全基因组DNA甲基化和mRNA表达谱确定了Kashin-Beck病和原发性骨关节炎之间的不同分子特征
7. Repetitive sequence analysis and karyotyping reveal different genome evolution and speciation of diploid and tetraploid Tripsacum dactyloides [O] . Qilin Zhu, Zexi Cai, Qilin Tang, 2016

机译：重复序列分析和核型分析揭示了二倍体和四倍体Tripsacum dactyloides的不同基因组进化和物种形成

Molecular verification of the integration of Tripsacum dactyloides DNA into wheat genome through wide hybridization

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