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首页> 外文期刊>Cytology and genetics >Expression Of Myelin Basic Protein And Glial Fibrillary Acidic Protein Genes In Human Glial Tumors
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Expression Of Myelin Basic Protein And Glial Fibrillary Acidic Protein Genes In Human Glial Tumors

机译：髓鞘碱性蛋白和胶质纤维酸性蛋白基因在人胶质瘤中的表达

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摘要

Analysis of the expression of genes encoding myelin basic protein (MBP) and glial fibrillary acidic protein (GFAP) in human glial tumors was carried out for determination of the expression specificity of these genes according to tumor types and their malignancy. Low levels of MBP mRNA in astrocytoma specimens of malignancy grades Ⅱ-Ⅳ and significantly higher levels in perifocal zones adjacent to them have been determined by Northern hybridization. Diffuse astrocytomas and anaplastic astrocytomas are characterized mostly by a low level of MBP gene expression and high level of GFAP gene expression, but distinct subtypes of diffuse and anaplastic astrocytomas with a high level of GFAP gene expression can also be detected that may be the reflection of different oncogenic pathways. Very low levels or even absence of MBP mRNA were revealed in oligodendroglioma and all oligoastrocytomas. Thus, Northern hybridization data are correlated with serial analysis of gene expression (SAGE). Obtained results show that MBP is a nonspecific marker for tumors of oligo-dendroglial origin, but determination of relative levels of MBP and GFAP mRNAs may be useful for glial tumor recognition. In such a way, these two genes together with YKL-40 and TSC-22, which we found previously, can be included into the gene panel for determination of so-called "gene signatures" of brain tumors. However, strict requirements in relation to a clinical value of these "gene signatures" cannot be formulated without verifying them on a large number of clinical samples of tumors and valid control.
机译：对人神经胶质瘤中编码髓鞘碱性蛋白（MBP）和神经胶质纤维酸性蛋白（GFAP）的基因表达进行分析，以根据肿瘤类型和恶性程度确定这些基因的表达特异性。通过Northern杂交可以确定，恶性级别为Ⅱ-Ⅳ的星形细胞瘤标本中的MBP mRNA含量较低，而邻近癌灶周围区域的MBP mRNA含量较高。弥漫性星形细胞瘤和间变性星形细胞瘤的主要特征是低水平的MBP基因表达和高水平的GFAP基因表达，但是也可以检测到具有高水平GFAP基因表达的弥漫性和间变性星形细胞瘤的不同亚型，这可能反映了不同的致癌途径。在少突胶质细胞瘤和所有少突星形胶质细胞瘤中发现了极低水平的MBP mRNA甚至不存在。因此，Northern杂交数据与基因表达的系列分析（SAGE）相关。获得的结果表明，MBP是少突胶质细胞来源肿瘤的非特异性标志物，但测定MBP和GFAP mRNA的相对水平可能对识别神经胶质瘤有用。以这种方式，我们先前发现的这两个基因与YKL-40和TSC-22一起可以被包括在基因组中，用于确定脑肿瘤的所谓“基因特征”。但是，如果不对大量的肿瘤临床样本和有效对照进行验证，就无法对这些“基因特征”的临床价值提出严格的要求。

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