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首页> 外文期刊>Biomaterials and Tissue Technology >Enzymatic crosslinking of Bombyx mori silk fibroin biomaterials: An investigation of the gel point by dynamic rheology
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Enzymatic crosslinking of Bombyx mori silk fibroin biomaterials: An investigation of the gel point by dynamic rheology

机译：家蚕丝素蛋白生物材料的酶促交联：通过动态流变学研究凝胶点

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摘要

Fibroin is the main polypeptidic component of the silk fibre generated by the larvae of the domesticated silk moth ( Bombyx mori ), and it has been extensively studied as a biomaterial with applications in tissue engineering and regenerative medicine. Due to their inherent practical advantages, the hydrogels constitute the preferred format for the B. mori silk fibroin (BMSF) biomaterials, which can conveniently be obtained by crosslinking processes. While the physically or chemically crosslinked BMSF hydrogels have been frequently described, the self-crosslinking of BMSF solutions induced by the catalytic effect of the enzyme horseradish peroxidase (HRP) has been barely reported. Following a previous preliminary study, where we demonstrated the advantages of using this enzyme for crosslinking BMSF, in the present work we investigated factors (amount of enzyme, initial fibroin concentration) that may affect the gelation. The measurement of dynamic moduli resulting from application of an oscillatory shear stress in a rheometer was our method to estimate the gelation time and to investigate the influence of certain factors on the process of crosslinking. It was found that both a higher initial concentration of the BMSF solution and a higher amount of the catalyst HRP induced a significant reduction of the gelation time.
机译：丝素蛋白是由家蚕（Bombyx mori）的幼虫产生的丝纤维的主要多肽成分，它已被广泛研究为生物材料，并在组织工程和再生医学中得到应用。由于其固有的实际优势，水凝胶构成了桑蚕丝素蛋白（BMSF）生物材料的优选形式，可以通过交联工艺方便地获得。虽然已经经常描述了物理或化学交联的BMSF水凝胶，但几乎没有报道过由辣根过氧化物酶（HRP）的催化作用引起的BMSF溶液的自交联。在先前的初步研究中，我们证明了使用该酶进行BMSF交联的优势，在本工作中，我们研究了可能影响凝胶化的因素（酶的量，初始纤维蛋白浓度）。流变仪中施加振荡剪切应力后产生的动态模量的测量是我们估算胶凝时间并研究某些因素对交联过程的影响的方法。已经发现，较高的BMSF溶液初始浓度和较高的催化剂HRP量均导致明显缩短了胶凝时间。

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