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首页> 外文期刊>BioResearch open access. >Transient Exposure to Hypoxic and Anoxic Oxygen Concentrations Promotes Either Osteogenic or Ligamentogenic Characteristics of PDL Cells
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Transient Exposure to Hypoxic and Anoxic Oxygen Concentrations Promotes Either Osteogenic or Ligamentogenic Characteristics of PDL Cells

机译：短暂暴露于低氧和缺氧氧浓度可促进PDL细胞的成骨或韧带形成特性

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The periodontal ligament (PDL) has a reservoir of mesenchymal stem cells (MSCs) and this tissue is easily available following teeth removal procedures. However, PDL-derived cells (PDLCs) availability for tissue engineering is limited because they are heterogeneous cells at various differentiation and lineage commitments. Therefore, efficient culture conditions to increase MSCs number are needed to use PDLCs in tissue engineering. Recent reports indicate that low-oxygen conditions amplified stem/progenitor cell numbers and inhibited cell differentiation. Our aim was to establish which low-oxygen culture conditions favored bone or tendon/ligament regeneration in cultured PDLCs. Human PDLCs were cultured and exposed to either hypoxic (O2≤5%) or anoxic (O2 oct4 , sox2 , p75 ) were upregulated after 6?h. At 24?h, these stem cell markers were maintained in PDLCs under hypoxic condition. Interestingly under anoxic conditions, expression of scleraxis gene (a key transcription factor for tendo/ligamentogenesis) was upregulated markedly. When hypoxic PDLCs were subcultured into osteogenic medium, in vitro calcification and prominent in vivo bone formation in mice calvaria were observed. When anoxic PDLCs were subcultured into tendo/ligamentogenic medium, expression of aggrecan (a mature tenogenic gene) increased remarkably. No obvious differences were detectable on chondrogenic and adipogenic inducibilities. We propose that transient exposure to low-oxygen during the culture enhanced MSC population in PDL. In addition, different low-oxygen concentrations favored osteogenic or tendo/ligamentogenic inducibilities of cultured PDLCs.
机译：牙周膜（PDL）具有间充质干细胞（MSCs）的储库，在拔除牙齿后即可轻松获得该组织。但是，由于PDL衍生细胞（PDLC）是具有各种分化和谱系承诺的异质细胞，因此可用于组织工程。因此，需要有效的培养条件来增加MSC的数量才能在组织工程中使用PDLC。最近的报道表明，低氧条件会扩增干/祖细胞数量并抑制细胞分化。我们的目标是确定哪种低氧培养条件有利于培养的PDLC中的骨骼或腱/韧带再生。培养人PDLC，并在6？h后上调至低氧（O 2 ≤5％）或缺氧（O 2 oct4，sox2，p75）。在24小时时，这些干细胞标记物在缺氧条件下保持在PDLC中。有趣的是，在缺氧条件下，巩膜基因（腱/韧带形成的关键转录因子）的表达明显上调。将缺氧PDLCs继代培养到成骨培养基中后，观察到小鼠颅骨的体外钙化和明显的体内骨骼形成。当将缺氧PDLCs继代培养到腱/韧带形成培养基中时，聚集蛋白聚糖（成熟的腱生基因）的表达显着增加。在软骨形成和脂肪形成诱导性上没有发现明显差异。我们建议在培养过程中短暂暴露于低氧会增强PDL中的MSC种群。另外，不同的低氧浓度有利于培养的PDLC的成骨或腱/韧带诱导。

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