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首页> 外文期刊>BioTechnology: An Indian Journal >Evaluation of 64oC and 76oC dry saunas as an environment for survival and growth of mesophilic and thermophilic bacteria
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Evaluation of 64oC and 76oC dry saunas as an environment for survival and growth of mesophilic and thermophilic bacteria

机译：评价64oC和76oC干桑拿房作为嗜温和嗜热细菌生存和生长的环境

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In this study, the diversity and variations of bacteria that were sampled from the floor of dry saunas operated at 64°C and 76°C for 6 days (Monday???Saturday) wereanalyzed to determineif the bacteria contaminating the dry saunas can grow, survive, or die. Bacteria sampled from the 64°C sauna grew at 45°C and 60°C but those from the 76°C sauna didnot growat either45°C or60°C. Sixteen and eightspecies of bacteria grewat 45°C and 60°C,respectively. Eight of 16 species grown at 45°C werethermophiles and all bacteria grown at 60oCwerethermophiles. DNA of non-growing bacteria sampled from a 76°C sauna wasdirectly extracted and analyzed by temperature gradient gel electrophoresis (TGGE). The non-growing bacteria included13 species based onthe band number of16S-rDNA separatedby TGGE. Thermophilescontaminating the64°C sauna were more frequently detected than mesophilesover the6 experimental days. Most of the mesophiles may have temporarily survivedand some of thermophilic bacteria survived for 4???5 days during sampling for the 6 days,whereas all bacteria contaminating the76°C sauna may lose their physiological function in a short time by drying out.
机译：在这项研究中，分析了从在64°C和76°C下运行6天（星期一至星期六）的干桑拿房地板采样的细菌的多样性和变异，以确定污染干桑拿房的细菌是否可以生长，生存或死亡。从64°C的桑拿浴中取样的细菌在45°C和60°C时生长，但从76°C的桑拿浴中细菌在45°C或60°C时均不生长。十六种和八种细菌分别在45°C和60°C下生长。在45°C下生长的16种物种中有8种是嗜热菌，所有细菌均在60°C的醚嗜热菌中生长。直接从76°C桑拿浴中取样的未生长细菌的DNA进行提取，并通过温度梯度凝胶电泳（TGGE）进行分析。基于TGGE分离的16S-rDNA的带数，非生长细菌包括13种。在6个实验日中，比中温细菌更频繁地检测到64°C桑拿中的嗜热菌。大多数嗜温菌可能已经暂时存活，并且某些嗜热细菌在取样的6天中存活了4×5天，而所有污染76°C桑拿浴的细菌都可能在短时间内因干燥而失去其生理功能。

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