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首页> 外文期刊>BioTechnology: An Indian Journal >Optimization of Polyhydroxyalkanoate Production by Recombinant E. coli Supplemented with Different Plant By-Products
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Optimization of Polyhydroxyalkanoate Production by Recombinant E. coli Supplemented with Different Plant By-Products

机译：补充不同植物副产物的重组大肠杆菌生产多羟基链烷酸酯的优化

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摘要

Polyhydroxyalkanoates (PHAs) are biodegradable polymers synthesized in cytoplasmic granules in bacteria, such as Cupriavidus necator (Ralstonia eutropha), and several other bacteria. PHAs accumulation occurs in response to stress conditions, i.e. under high carbon and low nitrogen (24:1 ratio). In this study, E. coli was genetically modified for PHA production in biofermentors. PHA was synthesized in bacteria transformed with the operon phbA/phbB/phbC. The bacteria were fed using a basal medium supplemented with three different plant by-products, potato tuber skin hydrolysate, corn hydrolysate, and banana juice supplement. The growth in biofermentor was monitored through the evaluation of consumption of sugars and quantification of PHA synthesis. A microarray scanner was used to read fluorescence intensity of Nile Blue stained bacteria. PHA production by E. coli fed on a banana juice supplement outperformed all the other fermentation media, with highest amount of PHA per dry cell weight.
机译：聚羟基链烷酸酯（PHA）是在细菌（例如铜杯菌（Ralstonia eutropha））和其他几种细菌的细胞质颗粒中合成的可生物降解的聚合物。在压力条件下，即在高碳和低氮（比例为24：1）下会发生PHAs积累。在这项研究中，对大肠杆菌进行了基因改造，以在生物发酵罐中生产PHA。 PHA是在用操纵子phbA / phbB / phbC转化的细菌中合成的。使用补充了三种不同植物副产物，马铃薯块茎皮水解产物，玉米水解产物和香蕉汁的基础培养基喂养细菌。通过评估糖的消耗和定量PHA合成来监测生物发酵罐中的生长。使用微阵列扫描仪读取尼罗蓝染色细菌的荧光强度。用香蕉汁补充剂喂养的大肠杆菌生产的PHA优于所有其他发酵培养基，每干细胞重量的PHA量最高。

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