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New side-view imaging technique for observing posterior chamber structures during cataract surgery in porcine eyes

机译:在猪眼白内障手术中观察后房结构的新型侧视成像技术

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Background To develop a side-view imaging technique for observing the dynamic behavior of posterior chamber structures (PCSs) in porcine eyes which mimics closed-eye cataract surgery in humans. Methods Enucleated porcine eyes were placed into liquid nitrogen for 5 seconds and immediately bisected at about a 45-degree angle to the equatorial plane. The anterior portion was attached firmly to a glass slide with superglue and sprinkled with wheat flour. Phacoemulsification and aspiration (PEA) was performed as in humans on 10 consecutive porcine eyes. The movements of the PCSs were monitored through the glass slide with a high-resolution video camera set below the cut surface of the eye. The intraocular pressure (IOP) was monitored during the surgery. The highest IOP, operation time, and volume of irrigation fluid of 10 whole eyes were compared to that obtained from the bisected eyes glued to a glass slide. In a second set of experiments, the strength of the seal between the bisected eye and the glass slide was tested in three sets of eyes: 1) frozen eye fixed with superglue with wheat flour for 3 min; 2) frozen eye fixed with superglue for 3 min; and 3) non-frozen eye fixed with superglue for 30 min. The highest IOP that led to a disruption of the seal was compared among the three groups. Results PEA was successfully performed on 9 of 10 (90%) eyes with the movements of the PCSs clearly observed. The average maximum intraocular pressure of the 9 bisected eyes was 55.8 ± 4.7 mmHg and that for the 10 unbisected eyes was 55.3 ± 5.0 mmHg (P = 0.650). The frozen eye fixed with superglue in combination with wheat flour (Group 1) had the strongest sealing strength with an average IOP at the breaking point of 117.3 ± 36.2 mmHg. Conclusions Our side-view imaging technique can be used to evaluate the changes of the PCSs during intraocular surgery and for surgical training of new residents.
机译:背景技术开发一种侧视图成像技术,以观察猪眼中后房结构(PCS)的动态行为,以模仿人类的闭眼白内障手术。方法将去核猪眼放在液氮中5秒钟,并立即以与赤道平面成45度角的方式将其一分为二。前面部分用强力胶牢固地附着在载玻片上,并撒上小麦粉。与人类一样,在连续的10只猪眼上进行超声乳化和抽吸(PEA)。 PCS的运动通过设置在眼睛切割面下方的高分辨率摄像机通过载玻片进行监控。在手术期间监测眼内压(IOP)。将10只全眼的最高IOP,手术时间和冲洗液量与粘在载玻片上的等分眼获得的最高眼压,手术时间和冲洗液进行了比较。在第二组实验中,在三组眼睛中测试了等分的眼睛和载玻片之间的密封强度:1)用超级胶和小麦粉固定3分钟的冷冻眼睛; 2)用强力胶固定冷冻眼3分钟; 3)用强力胶固定30分钟无冻眼。在三组中比较了导致密封破坏的最高眼压。结果在10眼中的9眼(90%)中成功进行了PEA,并且清楚地观察到PCS的运动。 9只平分的眼睛的平均最大眼压为55.8±4.7 mmHg,而10只平分的眼睛的平均最大眼压为55.3±5.0 mmHg(P = 0.650)。用强力胶和小麦粉(第1组)固定的冷冻眼具有最强的密封强度,平均IOP的断裂点为117.3±36.2 mmHg。结论我们的侧视成像技术可用于评估眼内手术期间PCS的变化以及对新居民的手术培训。

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