...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>BMC Bioinformatics >Algebraic correction methods for computational assessment of clone overlaps in DNA fingerprint mapping
【24h】

Algebraic correction methods for computational assessment of clone overlaps in DNA fingerprint mapping

机译：DNA指纹图谱中克隆重叠计算评估的代数校正方法

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background The Sulston score is a well-established, though approximate metric for probabilistically evaluating postulated clone overlaps in DNA fingerprint mapping. It is known to systematically over-predict match probabilities by various orders of magnitude, depending upon project-specific parameters. Although the exact probability distribution is also available for the comparison problem, it is rather difficult to compute and cannot be used directly in most cases. A methodology providing both improved accuracy and computational economy is required. Results We propose a straightforward algebraic correction procedure, which takes the Sulston score as a provisional value and applies a power-law equation to obtain an improved result. Numerical comparisons indicate dramatically increased accuracy over the range of parameters typical of traditional agarose fingerprint mapping. Issues with extrapolating the method into parameter ranges characteristic of newer capillary electrophoresis-based projects are also discussed. Conclusion Although only marginally more expensive to compute than the raw Sulston score, the correction provides a vastly improved probabilistic description of hypothesized clone overlaps. This will clearly be important in overlap assessment and perhaps for other tasks as well, for example in using the ranking of overlap probabilities to assist in clone ordering.
机译：背景Sulston分数是一个公认的指标，尽管是用于概率评估DNA指纹图谱中假定的克隆重叠的近似指标。众所周知，取决于项目特定的参数，系统会以各种数量级过高地预测匹配概率。尽管确切的概率分布也可用于比较问题，但计算起来相当困难，在大多数情况下不能直接使用。需要同时提供改进的准确性和计算经济性的方法。结果我们提出了一种简单的代数校正程序，该程序将Sulston分数作为暂定值，并应用幂律方程以获得改进的结果。数值比较表明，在传统琼脂糖指纹图谱典型参数范围内，准确性大大提高。还讨论了将方法外推到更新的基于毛细管电泳的项目的参数范围特征中的问题。结论尽管计算的费用仅比原始Sulston评分高出一点，但该修正大大改善了假设克隆重叠的概率描述。这在重叠评估中可能很重要，对于其他任务也可能很重要，例如，在使用重叠概率的排序来协助克隆排序时。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号