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首页> 外文期刊>British Biotechnology Journal >Effect of Arsenic (As) on the Spermatogenesis of Black Bengal Goat (Capra hircus) Reared at the Arsenic Prone Area of Mymensingh District in Bangladesh
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Effect of Arsenic (As) on the Spermatogenesis of Black Bengal Goat (Capra hircus) Reared at the Arsenic Prone Area of Mymensingh District in Bangladesh

机译：砷对孟加拉国Mymensingh区易生砷地区饲养的黑孟加拉山羊（Capra hircus）精子发生的影响

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Aim: Arsenic (As) alters the spermatogenic process as well as testicular histology has been reported in different species of domestic, wild, aquatic life, and laboratory animals. The present study was conducted to investigate the impact of toxicity of arsenic poison on the spermatogenesis of the Black Bengal goat at the most arsenic polluted area of Mymensingh district in Bangladesh by using histopathological techniques.Methods: A total of 12 adult Black Bengal male goats (Capra hircus) were used in this study. Among these, 6 were selected from the arsenic polluted area, and the rest were from the less contaminated area as control. Goats were sacrificed by using conventional animal killing method adopted in the laboratory. Both the right and left testes were collected aseptically. Testicular tissues were cut perpendicular to the long axis of the testis and preserved in Bouin’s solution. Paraffin block was made and tissue sections were cut at 5-μm in thickness. Tissues were processed for routine hematoxylin and eosin and Periodic Acid-Schiff (PAS)-hematoxylin stains. Thickness of tunica albugenia, spermatigenic cell layer, diameters of the seminiferous tubules, number of spermatogenic, sertoli, and leydig cells were counted and tabulated. Apoptotic spermatogenic cells were detected by using Apoptosis Detection Kit. The data collected was statistically analyzed for any significant differences between the arsenic exposed and control goats.Results: Our results revealed comparatively increased thickness of the tunica albugenia, wide inter-tubular spaces, low height of the spermatogenic cell layer, decreased diameter of the seminiferous tubules, decreased spermatogenic, sertoli, and leydig cell counts, and marked increased of apoptotic spermatogenic cells in the arsenic affected goats. The data differences between the arsenic affected and control goats were statistically significant (P<0.01).Conclusion: Our histopathological study revealed alteration of testicular tissues in arsenic affected goats. This morphological changes of testes significantly affected on the spermatogenic processes. But it was not possible to determine the possible stage of the spermatogenesis was interrupted by the arsenic. Decreased number of spermatogenic, sertoli, and leydig cell counts, and distinctly increased number of apoptotic spermatogenic cell indicates high toxic effects of arsenic poisoning on the male gonad. The mechanism of action of toxicity of the arsenic could not be understood clearly. It is suggested here that the Black Bengal goats can be experimentally used as animal model in the laboratory for investigating the role of arsenic on the reproduction of the domestic animals.
机译：目的：砷已经改变了生精过程以及睾丸组织学在不同种类的家养，野生，水生动物和实验动物中的报道。本研究采用组织病理学技术，研究了孟加拉国Mymensingh区砷污染最严重地区砷中毒对黑孟加拉山羊精子发生的影响。方法：共有12只成年黑孟加拉公山羊（在这项研究中使用了Capra hircus。其中，从砷污染区中选出6个，其余均从污染较轻的区中选出。使用实验室采用的常规动物杀死方法处死山羊。左右睾丸均无菌采集。垂直于睾丸长轴切开睾丸组织，并保存在布因溶液中。制作石蜡块并将组织切片切成5-μm的厚度。对组织进行常规苏木精和曙红和高碘酸希夫（PAS）-苏木精染色处理。计数并制表白衣的厚度，生精细胞层，生精小管的直径，生精细胞，sertoli细胞和leydig细胞的数量。使用凋亡检测试剂盒检测凋亡的生精细胞。结果：我们的结果表明，穿山甲的厚度相对增加，肾小管间间隙较宽，生精细胞层的高度较低，生精细胞的直径减小，因此对收集到的数据进行了统计学分析，以了解砷暴露山羊羊与对照山羊之间是否存在显着差异。在受砷影响的山羊中，小管，生精，睾丸和leydig细胞计数减少，并且凋亡的生精细胞明显增加。砷污染山羊与对照山羊之间的数据差异具有统计学意义（P <0.01）。结论：我们的组织病理学研究显示，砷污染山羊的睾丸组织发生了变化。睾丸的这种形态变化显着影响生精过程。但是不可能确定精子发生被砷中断的可能阶段。精子，精子和莱迪克细胞数量减少，凋亡的生精细胞数量明显增加，表明砷中毒对雄性腺有高毒性作用。砷的毒性作用机理尚不清楚。建议在实验室中将黑孟加拉山羊用作动物模型，以研究砷对家畜繁殖的作用。

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