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首页> 外文期刊>British Biotechnology Journal >Identification of Suitable Condition for MannanaseProduction by Bacillus sp. GA2(1)
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Identification of Suitable Condition for MannanaseProduction by Bacillus sp. GA2(1)

机译：确定芽孢杆菌属菌株生产甘露聚糖酶的合适条件GA2（1）

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Aims: The effects of 1% (w/v) supplementation of additional 5 agricultural wastes, corn cob, bagasses, coffee residues, soybean meal, and copra meal for mannanase production by Bacillus sp.GA2(1) were studied. Hence, partial characterization of mannanase was determined.Methodology: The 1%(v/v) overnight cultured of Bacillus sp. GA2(1) was transferred into the basal medium and shaken at 150 rpm for 18 h at 37oC. The additional of 5 AWs, corn cob, bagasses, coffee residues, soybean meal, and copra meal for the mannanase production were investigated. The cell suspension was centrifuged, and the crude mannanases were collected and stored at –20oC for enzyme assay. The mannanase activities were measured by the dinitrosalicylic acid method. The optimal pH of mannanase were studied by measuring enzyme activity at pH 3-10 using 50 mM of following buffers; citrate (pH 3.0-6.0), phosphate (pH 6.0-8.0), and glycine-NaOH (pH 8.0-10.0). The optimal temperature was measured at 30-80oC. Under standard assay conditions, locust bean gum was used as substrate to determine the optimal pH and temperature of the reaction. Thermostability was determined by preincubating the enzyme at different temperatures (30-80oC) for 1 h. The residual mannanase activities were measured under standard condition.Results: Among bagasses, coffee residues, soybean meal, corn cob and copra meal, the coffee residues was the most effective carbon source, the maximum yield of mannanase activity was 0.26 U/ml. The optimal temperature and pH for mannanase activity was pH 6.0 and 50oC of 0.44 and 0.35 U/ml, respectively. The stability of enzyme was determined at 30-80oC for 60 min. The results revealed that mannanase retained more than 96% of remaining activity after incubation of 60 min at 50oC.Conclusion: The maximum mannanase production was found when the medium was supplemented with coffee residues. Crude mannanase showed the highest activities of 0.44 U/ml at pH 6.0 and of 0.35 U/ml at 50oC. The mannanase from Bacillus sp. GA2(1) retained more than 90% of theirs activities at 30-60oC after preincubated for 60 min and then rapidly decreased.
机译：目的：研究了添加1％（w / v）的另外5种农业废料，玉米芯，蔗渣，咖啡渣，豆粕和椰菜粕对芽孢杆菌GA2（1）生产甘露聚糖酶的影响。因此，确定了甘露聚糖酶的部分表征。方法：芽孢杆菌属菌株的1％（v / v）过夜培养。将GA2（1）转移至基础培养基中，并在37oC下以150 rpm的转速振摇18 h。研究了另外5种AW，玉米芯，蔗渣，咖啡渣，豆粕和椰干粕用于甘露聚糖酶的生产。离心细胞悬液，收集粗制的甘露聚糖酶并保存在–20oC进行酶分析。甘露聚糖酶活性通过二硝基水杨酸法测定。通过使用50 mM以下缓冲液在pH 3-10下测量酶活性来研究甘露聚糖酶的最佳pH。柠檬酸盐（pH 3.0-6.0），磷酸盐（pH 6.0-8.0）和甘氨酸NaOH（pH 8.0-10.0）。最佳温度为30-80oC。在标准测定条件下，刺槐豆胶用作底物，以确定反应的最佳pH和温度。通过将酶在不同温度（30-80oC）下预孵育1小时来确定热稳定性。结果：在蔗渣，咖啡渣，豆粕，玉米芯和椰干粕中，咖啡渣是最有效的碳源，甘露聚糖酶活性的最大产量为0.26U / ml。甘露聚糖酶活性的最佳温度和pH分别为6.0和50oC，分别为0.44和0.35 U / ml。在30-80oC下60分钟测定酶的稳定性。结果表明，甘露聚糖酶在50oC下孵育60分钟后保留了超过96％的剩余活性。结论：当培养基中添加咖啡残留物时，甘露聚糖酶的产量最高。粗甘露聚糖酶在pH 6.0时显示最高活性，为0.44 U / ml，在50oC时显示最高活性，为0.35 U / ml。来自芽孢杆菌属的甘露聚糖酶。预孵育60分钟后，GA2（1）在30-60oC时保留了90％以上的活性，然后迅速下降。

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