The experiment screened three strains (Bacillus subtilis 4.37,Bacillus subtilis Coland w-031 3.83,Bacillus licheniformis 3.83) with highest enzyme activity from seven kinds of Bacillus with the method of medium transparent circle.The three strains were rescreened by measuring the enzyme activity of the liquid fermentation.As a result the Bacillus has the highest enzyme activity.The fermentation conditions were optimized by single factor experiment and the optimum fermentation conditions were as follows:konjac flour is the best carbon source and the best content is 4%.The best organic nitro gen source and inorganic nitro gen source are yeast extract and sodium nitrate .The best enzyme production time is 36 h.%本实验用培养基透明圈法从7种芽孢杆菌中筛选到酶活最高的三株芽孢杆菌（枯草芽孢杆菌4．37、高龙枯草芽孢杆菌w-0313．83、地衣芽孢杆菌3．83）。用测定发酵液酶活的方法进行菌株的复筛，酶活最高的菌株为枯草芽孢杆菌。对该菌产酶条件进行了单因素实验发酵条件的优化，最优产酶条件为：最佳碳源是魔芋粉，含量为4％，最佳有机氮源为酵母浸粉，最佳无机氮源为硝酸钠，最佳产酶时间为36 h。
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