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首页> 外文期刊>British Biotechnology Journal >Antimicrobial Resistance and Detection of Biofilm in Staphylococcus aureus Isolates from Casablanca
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Antimicrobial Resistance and Detection of Biofilm in Staphylococcus aureus Isolates from Casablanca

机译：卡萨布兰卡金黄色葡萄球菌分离株的抗药性及生物膜检测

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Aims: The ability of biofilm formation seems to play an essential role in the virulence of Staphylococcus aureus . The aims of the present study were to test the sensitivity of the clinical isolates of Staphylococcus aureus to antibiotics, detect the ability of these strains to form biofilm and evaluate the correlation between biofilm formation by clinical isolates and the resistance to antibiotics. Place and Duration of Study: Laboratory of Virology, Microbiology and Quality/ Eco-toxicology and Biodiversity, Faculty of Sciences and Techniques Mohammedia, University Hassan II Casablanca and laboratory of Bacteriology, Virology and Hygiene, Ibn Rochd University Hospital, Casablanca during October 2015 and January 2016. Methodology: A total of 117 clinical isolates of staphylococci were collected at the University Hospital Ibn Rochd of Casablanca, Morocco and examined for antimicrobial susceptibility, presence of mecA gene and biofilm formation. Staphylococci species identification and antibiogram were performed by standard procedures using disk diffusion method. The methicillin resistance was confirmed by PCR using mecA specific primers. The biofilm formation assay was realised by the tissue culture plate method (TCP). Results: Among all strains collected, 74 were identified as Staphylococcus aureus . Out of 74 Staphylococcus aureus , 22 strains (29.7 %) were found methicillin resistant when tested with cefoxitin disc diffusion method. 20.3 %, 18.9 % and 13.5 % were classified as resistant to kanamicin, ciprofloxacin and erythromycin respectively. All strains were found resistant to penicillin G and sensitive to teicoplanin. All isolates resistant to methicillin by cefoxitin disc diffusion method were confirmed by presence the mecA gene by PCR. Of the 74 isolates 16 (21.6 %) were non adherent, 40 (54 %) weakly adherent, 12 (16.2 %) moderately adherent and 6 (8.1 %) strongly adherent. Conclusion: The results of this study showed that there is a correlation between biofilm formation and resistance to all the antibiotics tested, except to teicoplanin, which was active against the all strains.
机译：目的：生物膜形成的能力似乎在金黄色葡萄球菌的毒性中起着至关重要的作用。本研究的目的是测试金黄色葡萄球菌临床分离株对抗生素的敏感性，检测这些菌株形成生物膜的能力，并评估临床分离株形成生物膜与对抗生素的耐药性之间的相关性。研究的地点和持续时间：卡萨布兰卡哈桑二世大学Mohammedia理工学院病毒学，微生物学和质量/生态毒理学和生物多样性实验室以及卡萨布兰卡伊本·罗奇大学医院细菌学，病毒学和卫生学实验室2016年1月。方法：在摩洛哥卡萨布兰卡的伊本·罗希德大学医院共收集了117株葡萄球菌临床分离株，并检查了其药敏性，mecA基因的存在和生物膜的形成。葡萄球菌的种类鉴定和抗菌素检测是通过标准方法，使用圆盘扩散法进行的。使用mecA特异性引物通过PCR确认了甲氧西林抗药性。通过组织培养板法（TCP）实现生物膜形成测定。结果：在收集的所有菌株中，有74株被鉴定为金黄色葡萄球菌。用头孢西丁圆盘扩散法检测时，在74株金黄色葡萄球菌中，发现22株（29.7％）耐甲氧西林。分别对卡那霉素，环丙沙星和红霉素有抗药性的分别为20.3％，18.9％和13.5％。发现所有菌株对青霉素G具有抗性并且对替考拉宁敏感。通过头孢西丁圆盘扩散法对耐甲氧西林的所有菌株均通过PCR检测到存在mecA基因而得到证实。在74个分离株中，有16个（21.6％）是非粘附性的，有40个（54％）是弱粘附性的，有12个（16.2％）是中度粘附的，有6个（8.1％）是强粘附性的。结论：这项研究的结果表明，生物膜的形成与对所有测试抗生素的耐药性相关，除了替考拉宁对所有菌株均具有活性。

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