• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Cell Division >Antiproliferative and cell apoptosis-inducing activities of compounds from Buddleja davidii in Mgc-803 cells
【24h】

Antiproliferative and cell apoptosis-inducing activities of compounds from Buddleja davidii in Mgc-803 cells

机译：蟾蜍化合物对Mgc-803细胞的抗增殖和诱导细胞凋亡活性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background Buddleja davidii is widely distributed in the southwestern region of China. We have undertaken a systematic analysis of B. davidii as a Chinese traditional medicine with anticancer activity by isolating natural products for their activity against the human gastric cancer cell line Mgc-803 and the human breast cancer cell line Bcap-37. Results Ten compounds were extracted and isolated from B. davidii, among which colchicine was identified in B. davidii for the first time. The inhibitory activities of these compounds were investigated in Mgc-803, Bcap-37 cells in vitro by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, and the results showed that luteolin and colchicine had potent inhibitory activities against the growth of Mgc-803 cells. Subsequent fluorescence staining and flow cytometry analysis indicated that these two compounds could induce apoptosis in Mgc-803 cells. The results also showed that the percentages of early apoptotic cells (Annexin V+/PI-, where PI is propidium iodide) and late apoptotic cells (Annexin V+/PI+) increased in a dose- and time-dependent manner. After 36 h of incubation with luteolin at 20 μM, the percentages of cells were approximately 15.4% in early apoptosis and 43.7% in late apoptosis; after 36 h of incubation with colchicine at 20 μM, the corresponding values were 7.7% and 35.2%, respectively. Conclusions Colchicine and luteolin from B. davidii have potential applications as adjuvant therapies for treating human carcinoma cells. These compounds could also induce apoptosis in tumor cells.
机译：背景蟾蜍（Buddleja davidii）在中国西南地区广泛分布。我们通过分离天然产物对人胃癌细胞系Mgc-803和人乳腺癌细胞系Bcap-37的活性，对具有抗癌活性的中草药B. davidii进行了系统分析。结果从大卫氏芽孢杆菌中提取并分离出十种化合物，其中首次在大卫氏芽孢杆菌中鉴定了秋水仙碱。通过MTT [3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑鎓溴化物]测定，在体外Mgc-803，Bcap-37细胞中研究了这些化合物的抑制活性，结果表明：木犀草素和秋水仙碱对Mgc-803细胞的生长具有有效的抑制作用。随后的荧光染色和流式细胞仪分析表明这两种化合物可以诱导Mgc-803细胞凋亡。结果还表明，早期凋亡细胞（Annexin V + / PI -，其中PI为碘化丙啶）和晚期凋亡细胞（Annexin V + / PI +）的百分比增加以剂量和时间依赖的方式。与木犀草素在20μM下孵育36小时后，早期凋亡的细胞百分比约为15.4％，晚期凋亡的细胞百分比约为43.7％;与20μM秋水仙碱孵育36小时后，相应值分别为7.7％和35.2％。结论大卫氏芽孢杆菌的秋水仙碱和木犀草素作为治疗人类癌细胞的辅助疗法具有潜在的应用前景。这些化合物还可以诱导肿瘤细胞凋亡。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号