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首页> 外文期刊>Cellular Physiology and Biochemistry >1α,25-Dihydroxycholecalciferol (Vitamin D3) Induces NO-Dependent Endothelial Cell Proliferation and Migration in a Three-Dimensional Matrix
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1α,25-Dihydroxycholecalciferol (Vitamin D3) Induces NO-Dependent Endothelial Cell Proliferation and Migration in a Three-Dimensional Matrix

机译：1α，25-二羟基胆钙化固醇（维生素D3）诱导NO依赖性内皮细胞在三维基质中的增殖和迁移

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biBackground/Aims /i/bThe 1α,25-dihydroxycholecalciferol (Vit. D) induces eNOS dependent nitric oxide (NO) production in human umbilical vein endothelial cells (HUVEC). To our knowledge, there are no reports directly relating Vit. D induced NO production to proliferation and/or migration in endothelial cells (EC). The aim of this study was to evaluate whether Vit. D addition to porcine EC could affect their proliferation and/or migration in a three-dimensional matrix via NO production. biMaterials and Methods /i/bPorcine aortic endothelial cells (PAE) were used to evaluate Vit. D effects on cell proliferation and migration in a three-dimensional matrix. biResults /i/bVit. D induced NO production in PAE cells. Moreover, it induced a significant increase in cellular proliferation and migration in a three-dimensional matrix. These effects were NO dependent, as inhibiting eNOS activity by L-NAME PAE migration was abrogated. This effect was strictly related to MMP-2 expression and apparently dependent on Vit. D and NO production. biConclusions /i/bVit. D can promote both endothelial cells proliferation and migration in a three-dimensional matrix via NO-dependent mechanisms. These findings cast new light on the role of Vit. D in the angiogenic process, suggesting new applications for Vit. D in such fields as tissue repair and wound healing.
机译：背景/目的 1α，25-二羟基胆钙化固醇（Vit。D）诱导人脐静脉内皮细胞（HUVEC）产生eNOS依赖的一氧化氮（NO）。据我们所知，尚无与Vit直接相关的报告。 D在内皮细胞（EC）中诱导NO产生以增殖和/或迁移。这项研究的目的是评估是否Vit。猪EC中的D可能通过NO的产生影响其在三维矩阵中的增殖和/或迁移。 材料和方法 使用猪主动脉内皮细胞（PAE）评估Vit。 D在三维矩阵中对细胞增殖和迁移的影响。 结果 Vit。 D诱导PAE细胞中NO的产生。而且，它在三维矩阵中诱导了细胞增殖和迁移的显着增加。这些作用不受NO影响，因为废除了L-NAME PAE迁移对eNOS的抑制作用。该作用与MMP-2表达严格相关，并且显然依赖于Vit。 D和没有生产。 结论 Vit。 D可以通过NO依赖性机制促进内皮细胞在三维矩阵中的增殖和迁移。这些发现为Vit的作用提供了新的线索。 D在血管生成过程中，提示Vit的新应用。 D在组织修复和伤口愈合等领域。

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