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A New Role for Aquaporin 7 in Insulin Secretion

机译：水通道蛋白7在胰岛素分泌中的新作用

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Bacgrouns/Aims Several insulinotropic agents were recently reported to cause β-cell swelling. The possible participation of AQP7 to water transport was investigated in AQP7sup+/ +/sup or AQP7sup-/-/sup mice. Methods Aquaporin expression, insulin secretion, cell swelling and electrical activity were investigated in pancreatic islets. Results RT-PCR revealed the expression of AQP5 and AQP8 mRNA. Double immunofluorescent labeling indicated their presence in β-cells. Whilst basal insulin release from isolated pancreatic islets incubated at 2.8 mM D-glucose did not differ between AQP7sup+/ +/sup or AQP7sup-/-/sup mice, the secretion of insulin evoked by the omission of 50 mM NaCl, the substitution of 50 mM NaCl by 100 mM glycerol or a rise in D-glucose concentration to 8.3 mM and 16.7 mM was severely impaired in the islets from AQP7sup-/-/sup mice. Yet, exposure of β-cells to either the hypotonic medium or a rise in D-glucose concentration caused a similar degree of swelling and comparable pattern of electrical activity in cells from AQP7sup+/ +/sup and AQP7sup-/-/sup mice. Both the cell swelling and change in membrane potential were only impaired in AQP7sup-/-/sup cells when exposed to 50 mM glycerol. Conclusion It is proposed, therefore, that AQP7 may, directly or indirectly, play a role at a distal site in the exocytotic pathway.
机译：Bacgrouns /目的最近有报道称几种促胰岛素药会引起β细胞肿胀。在AQP7 + / + 或AQP7 -/-小鼠中研究了AQP7可能参与水运输。方法研究胰岛中水通道蛋白的表达，胰岛素分泌，细胞肿胀和电活动。结果RT-PCR显示AQP5和AQP8 mRNA的表达。双重免疫荧光标记表明它们存在于β细胞中。 AQP7 + / + 或AQP7 -/-小鼠在分离的胰岛中从2.8 mM D-葡萄糖培养的基础胰岛素释放无差异，胰岛素诱发的分泌由在AQP7 -/-小鼠的胰岛中，缺失50 mM NaCl，用100 mM甘油替代50 mM NaCl或D-葡萄糖浓度升高至8.3 mM和16.7 mM受到严重损害。 。但是，β细胞暴露于低渗介质或D-葡萄糖浓度升高会导致AQP7 + / + 和AQP7 -/-小鼠。仅在暴露于50 mM甘油的AQP7 -/-细胞中，细胞肿胀和膜电位的变化才受到损害。结论因此，建议AQP7可以直接或间接在胞吐途径的远端部位发挥作用。

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