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首页> 外文期刊>CHROMATOGRAPHY >Determination of Amino Acids in Human Pancreas Tissue Sections Using Liquid Chromatography Tandem Mass Spectrometry
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Determination of Amino Acids in Human Pancreas Tissue Sections Using Liquid Chromatography Tandem Mass Spectrometry

机译：液相色谱-串联质谱法测定人胰腺组织切片中的氨基酸

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摘要

A new analytical method was developed for the determination of 35 different amino acids in human pancreas tissue sections embedded in O.C.T. by liquid chromatography tandem mass spectrometry with precolumn derivatization. An AccQ-Tag was selected as a derivatization reagent because it could avoid the ion suppression caused by matrix compounds in the pancreas tissue. This new method uses two different internal standards during the pretreatment processes to achieve good accuracy and high sensitivity, as well as a multiple reaction monitoring time segment program during the acquisition process. This method was fully validated for 35 different amino acids and showed a good linearity of calibration curve (r~(2)>0.993), with a lower limit of quantification in the range of 0.01–0.75 μM. Furthermore, this method was applied to the analysis of 35 different amino acids in human pancreas tissue sections, and successfully allowed for the determination of 27 of these compounds. The accuracy of our newly developed method was estimated to be in the range of 87%–115% based on the results of a recovery test. This method therefore represents a powerful analytical platform for the analysis of human tissue sections and could be used to investigate the mechanism of pancreatic disease.
机译：开发了一种新的分析方法，用于测定O.C.T中包埋的人胰腺组织切片中的35种不同氨基酸。液相色谱串联质谱联用前柱衍生化。选择AccQ-Tag作为衍生试剂是因为它可以避免胰腺组织中基质化合物引起的离子抑制。这种新方法在预处理过程中使用了两种不同的内标，以实现良好的准确性和高灵敏度，并在采集过程中使用了多反应监测时间段程序。该方法针对35种不同氨基酸进行了充分验证，并显示出良好的校准曲线线性（r〜（2）> 0.993），定量下限在0.01–0.75μM之间。此外，该方法还用于分析人胰腺组织切片中的35种不同氨基酸，并成功地测定了其中27种化合物。根据恢复测试的结果，我们新开发的方法的准确性估计在87％–115％的范围内。因此，该方法代表了用于分析人体组织切片的强大分​​析平台，可用于研究胰腺疾病的机制。

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