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Mutator-Like Elements with Multiple Long Terminal Inverted Repeats in Plants

机译:植物中具有多个长末端倒置重复序列的突变因子样元件

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Mutator-like transposable elements (MULEs) are widespread in plants and the majority have long terminal inverted repeats (TIRs), which distinguish them from other DNA transposons. It is known that the long TIRs ofMutatorelements harbor transposase binding sites and promoters for transcription, indicating that the TIR sequence is critical for transposition and for expression of sequences between the TIRs. Here, we report the presence of MULEs with multiple TIRs mostly located in tandem. These elements are detected in the genomes of maize, tomato, rice, andArabidopsis. Some of these elements are present in multiple copies, suggesting their mobility. For those elements that have amplified, sequence conservation was observed for both of the tandem TIRs. For one MULE family carrying a gene fragment, the elements with tandem TIRs are more prevalent than their counterparts with a single TIR. The successful amplification of this particular MULE demonstrates that MULEs with tandem TIRs are functional in both transposition and duplication of gene sequences.
机译:突变体样转座因子(MULE)在植物中广泛分布,并且大多数具有长末端反向重复序列(TIR),这使它们与其他DNA转座子区分开。已知诱变元件的长TIR具有转座酶结合位点和用于转录的启动子,这表明TIR序列对于TIR之间的转位和序列表达是至关重要的。在这里,我们报告存在多个TIR串联的MULE。这些元素在玉米,番茄,水稻和拟南芥的基因组中检测到。这些元素中的一些以多份形式存在,表明它们具有流动性。对于那些已扩增的元件,两个串联TIR均观察到了序列保守性。对于一个携带基因片段的MULE家族,具有串联TIR的元件比具有单个TIR的元件更普遍。此特定MULE的成功扩增表明,带有串联TIR的MULE在基因序列的转座和复制中均起作用。

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