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首页> 外文期刊>Comparative and functional genomics >The Analysis of Multiple Genome Comparisons in GenusEscherichiaand Its Application to the Discovery of Uncharacterised Metabolic Genes in UropathogenicEscherichia coliCFT073
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The Analysis of Multiple Genome Comparisons in GenusEscherichiaand Its Application to the Discovery of Uncharacterised Metabolic Genes in UropathogenicEscherichia coliCFT073

机译：大肠埃希氏菌的多个基因组比较分析及其在发现致病性大肠埃希氏菌中未鉴定的代谢基因中的应用

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摘要

A survey of a complete gene synteny comparison has been carried out between twenty fully sequenced strains from the genusEscherichiawith the aim of finding yet uncharacterised genes implicated in the metabolism of uropathogenic strains ofE. coli(UPEC). Several sets of adjacent colinear genes have been identified which are present in all four UPEC included in this study (CFT073, F11, UTI89, and 536), annotated with putative metabolic functions, but are not found in any other strains considered. An operon closely homologous to that encoding the L-sorbose degradation pathway inKlebsiella pneumoniaehas been identified inE. coliCFT073; this operon is present in all of the UPEC considered, but only in 7 of the other 16 strains. The operon's function has been confirmed by cloning the genes intoE. coliDH5αand testing for growth on L-sorbose. The functional genomic approach combining in silico and in vitro work presented here can be used as a basis for the discovery of other uncharacterised genes contributing to bacterial survival in specific environments.
机译：已经在来自埃希氏菌属的二十个完全测序的菌株之间进行了完整的基因同源性比较的调查，目的是发现尚未鉴定的与E的尿路致病性菌株的代谢有关的基因。大肠杆菌（UPEC）。已鉴定出几组相邻的共线性基因，这些基因存在于本研究中的所有四个UPEC中（CFT073，F11，UTI89和536），并带有假定的代谢功能，但在考虑的任何其他菌株中均未发现。在E中鉴定到了与编码肺炎克雷伯菌中L-山梨糖降解途径的操纵子非常相似的操纵子。 coliCFT073;该操纵子存在于所考虑的所有UPEC中，但仅存在于其他16个菌株中的7个中。操纵子的功能已通过将基因克隆到E中得以证实。并检测L-山梨糖的生长。本文介绍的结合计算机和体外工作的功能基因组学方法可以用作发现其他在特定环境中有助于细菌存活的未表征基因的基础。

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