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首页> 外文期刊>Clinical and diagnostic laboratory immunology >A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay)
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A Latex Bead-Based Flow Cytometric Immunoassay Capable of Simultaneous Typing of Multiple Pneumococcal Serotypes (Multibead Assay)

机译：基于乳胶珠的流式细胞免疫测定能够同时分型多种肺炎球菌血清型（多珠测定）

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A simple and rapid method of simultaneously determining 15Streptococcus pneumoniae serotypes was developed. Fifteen latex beads of different sizes and different red fluorescence levels were coated with 1 of 15 serotypes (1, 3, 4, 5, 6A, 6B, 7F, 9N, 9V, 14, 18C, 19A, 19F, 22F, and 23F) of pneumococcal capsular polysaccharide (PS). The bead mixture was incubated with individual pneumococcal lysate, a pool of rabbit antisera capable of binding the 15 serotypes, and fluorescein (green fluorescence)-conjugated anti-rabbit antibody. Bead size, red fluorescence, and green fluorescence were measured in a single flow cytometer run. The green fluorescence of the beads was inhibited only when there was a serotypic match between PS on the bead and PS in the pneumococcal lysate. This method distinguished cross-reactive serotypes and correctly identified the serotypes in 100% of 86 pneumococcal isolates tested.
机译：建立了同时测定15种肺炎链球菌血清型的简便快速方法。用15种血清型（1、3、4、5、6A，6B，7F，9N，9V，14、18C，19A，19F，22F和23F）中的15种涂覆不同大小和不同红色荧光水平的15个乳胶珠肺炎球菌荚膜多糖（PS）。将珠混合物与单独的肺炎球菌裂解物，能够结合15种血清型的兔抗血清库和荧光素（绿色荧光）缀合的抗兔抗体一起孵育。在单个流式细胞仪中测量珠子大小，红色荧光和绿色荧光。仅当珠粒上的PS和肺炎球菌裂解液中的PS发生血清型匹配时，珠粒的绿色荧光才被抑制。该方法区分了交叉反应血清型，并正确鉴定了86种肺炎球菌分离株中100％的血清型。

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