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首页> 外文期刊>European journal of clinical microbiology and infectious diseases: Official publication of the European Society of Clinical Microbiology >Development of a multiplexed bead-based immunoassay for the simultaneous detection of antibodies to 17 pneumococcal proteins.
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Development of a multiplexed bead-based immunoassay for the simultaneous detection of antibodies to 17 pneumococcal proteins.

机译：用于同时检测针对17种肺炎球菌蛋白的抗体的基于多重磁珠的免疫测定方法的开发。

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Presently, several pneumococcal proteins are being evaluated as potential vaccine candidates. Here, we gather novel insights in the immunogenicity of PLY, PsaA, PspA, PspC, NanA, Hyl, PpmA, SlrA, Eno, IgA1-protease, PdBD, BVH-3, SP1003, SP1633, SP1651, SP0189 and SP0376. We developed a multiplex bead-based immunoassay (xMAP((R)) Technology, Luminex Corporation) to simultaneously quantify antibodies against these 17 pneumococcal proteins in serum. The median fluorescence intensity (MFI) values obtained for human pooled serum with the multiplex assay were between 82% and 111% (median 94%) of those obtained with the singleplex assays. For IgG, the coefficient of variation (CV) in serum ranged from 2% to 9%, for IgA, the CV ranged from 3% to 14% and for IgM, the CV ranged from 11% to 15%. Using this immunoassay, we showed that anti-pneumococcal antibody levels exhibited extensive inter-individual variability in young children suffering from invasive pneumococcal disease. All proteins, including the proteins with, as yet, unknown function, were immunogenic. In conclusion, the multiplex Streptococcus pneumoniae immunoassay based on proteins is reproducible. This assay can be used to monitor anti-S. pneumoniae antibody responses in a material- and time-saving manner.
机译：目前，几种肺炎球菌蛋白正在被评估为潜在的疫苗候选物。在这里，我们收集了有关PLY，PsaA，PspA，PspC，NanA，Hyl，PpmA，SlrA，Eno，IgA1-蛋白酶，PdBD，BVH-3，SP1003，SP1633，SP1651，SP0189和SP0376的免疫原性的新颖见解。我们开发了基于多重珠的免疫测定法（Luminex Corporation的xMAP（R）技术），以同时量化针对血清中这17种肺炎球菌蛋白质的抗体。通过多重测定获得的人类混合血清的中值荧光强度（MFI）值介于通过单一多重测定获得的中值的82％至111％（中值94％）之间。对于IgG，血清中的变异系数（CV）为2％至9％，对于IgA，CV为3％至14％，对于IgM，CV为11％至15％。使用该免疫测定法，我们显示抗肺炎球菌抗体水平在患有浸润性肺炎球菌疾病的幼儿中表现出广泛的个体间差异。所有蛋白质，包括功能未知的蛋白质都是免疫原性的。总之，基于蛋白质的多重肺炎链球菌免疫测定是可重复的。该测定法可用于监测抗S。肺炎抗体反应可节省材料并节省时间。

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