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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Genetic Variation at the Mitochondrial Large-Subunit rRNA Locus of Pneumocystis Isolates from Simian Immunodeficiency Virus-Infected Rhesus Macaques
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Genetic Variation at the Mitochondrial Large-Subunit rRNA Locus of Pneumocystis Isolates from Simian Immunodeficiency Virus-Infected Rhesus Macaques

机译：猿猴免疫缺陷病毒感染恒河猴的肺孢子虫线粒体大亚基rRNA基因座的遗传变异。

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摘要

The nucleotide sequences of a segment of the Pneumocystis mitochondrial large-subunit (mt LSU) rRNA gene from rhesus macaques coinfected with simian immunodeficiency virus (SIV) and Pneumocystis carinii were examined. Of 12 isolates examined, 3 were found to be identical and the others showed substantial sequence variation, with up to 13% divergence among variants. We identified two general sequence types that differed at several sites, including a conserved 26-nucleotide insertion. Four monkeys had evidence of two Pneumocystis variants present simultaneously, indicative of a mixed infection. There was a high degree of variance between the rhesus macaque-derived Pneumocystis mt LSU rRNA gene sequence and the cognate sequences in Pneumocystis organisms derived from other hosts. Analysis of the mt LSU rRNA genes of Pneumocystis organisms derived from rhesus macaques and several other mammalian hosts supports the observation that rhesus macaque-derived Pneumocystis is most closely related to human-derived Pneumocystis. In addition, the data identify the mt LSU rRNA gene as an informative locus for transmission and epidemiological studies of the SIV-rhesus macaque model of Pneumocystis infection.
机译：检测了与猿猴免疫缺陷病毒（SIV）和卡氏肺孢子虫（Pneumocystis carinii）共感染的恒河猴的肺孢子虫线粒体大亚基（mt LSU）rRNA基因片段的核苷酸序列。在检查的12个分离株中，发现3个是相同的，其他分离株显示出实质的序列变异，变异之间的差异高达13％。我们鉴定了在几个位点不同的两种通用序列类型，包括保守的26个核苷酸插入。四只猴子有两个同时存在的 Pneumocystis 变体的证据，表明混合感染。恒河猴来源的肺囊虫 mt LSU rRNA基因序列与其他宿主的肺囊虫生物的同源序列之间存在高度差异。对恒河猴和其他几种哺乳动物宿主的肺孢子虫微生物的mt LSU rRNA基因的分析支持以下观察：恒河猴衍生的肺孢子虫与人类来源最密切相关肺囊肿。此外，数据确定了mt LSU rRNA基因是肺囊虫感染的SIV-猕猴模型的传播和流行病学研究的信息源。

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