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首页> 外文期刊>Clinical and diagnostic laboratory immunology >Immunoglobulin G, A, and M Responses in Serum and Circulating Immune Complexes Elicited by the 16-Kilodalton Antigen of Mycobacterium tuberculosis
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Immunoglobulin G, A, and M Responses in Serum and Circulating Immune Complexes Elicited by the 16-Kilodalton Antigen of Mycobacterium tuberculosis

机译：结核分枝杆菌的16-Kilodalton抗原引起的血清和循环免疫复合物中的免疫球蛋白G，A和M反应

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摘要

The 16-kDa cytosolic antigen of M. tuberculosis was purified to homogeneity by molecular sieving chromatography, and the diagnostic potential of the antigen was evaluated in various categories of patients by enzyme-linked immunosorbent assay (ELISA). The immunoglobulin G (IgG), IgA, and IgM antibody levels to 16-kDa antigen were estimated in the two polar groups, namely, smear- and culture-positive pulmonary tuberculosis (S+C+) patients and healthy subjects (HS). Sensitivities of 62, 52 and 11% with specificities of 100, 97, and 95% were obtained for the three isotypes, respectively. The total number of positives by a combination of the three isotypes was analyzed in the polar groups, and the sensitivity improved to 83% with a specificity of 93%. Even when a combination of IgG and IgA alone was considered, the sensitivity was 82% with a specificity of 97%. Polyethylene glycol precipitation of the circulating immune complex (CIC) in sera was carried out. The CIC-bound antibodies to 16-kDa antigen were assessed by ELISA in the S+C+, S?C+, and S?C? categories of patients. Measuring the IgG-IgA-IgM combination positivities of the CIC-bound antibodies gave sensitivities of 97.5, 100, and 45.3%, respectively. The specificity of the assay with these combinations was maintained at 95.4%.
机译：em的16kDa胞质抗原。通过分子筛层析纯化结核病至同质，并通过酶联免疫吸附试验（ELISA）评估抗原在各种类型患者中的诊断潜力。在两个极地组，即涂片阳性和培养阳性的肺结核（S + C + ）患者和健康受试者（HS）。三种同种型分别获得62、52和11％的敏感性，特异度分别为100、97和95％。在极性组中分析了三种同种型组合的阳性总数，灵敏度提高到83％，特异性为93％。即使仅考虑IgG和IgA的组合，敏感性仍为82％，特异性为97％。进行了血清中循环免疫复合物（CIC）的聚乙二醇沉淀。通过ELISA在S + C + ，S ？ C + < / sup>和S ？ C ？患者类别。测量CIC结合抗体的IgG-IgA-IgM组合的阳性率，分别得出97.5％，100％和45.3％的灵敏度。用这些组合测定的特异性保持在95.4％。

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