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Indigenous production of biosurfactant and degradation of crude oil

机译：生物表面活性剂的土著生产和原油降解

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The present study investigated the isolation and identification of biosurfactant producing bacteria from Iranian oil wells. The biosurfactant production of bacteria isolates was evaluated and confirmed using hemolysis and emulsification tests. The biodegradation of crude oil was studied using GC and HPLC analysis. A total of 45 strains have been isolated. These strains showed less than a 40 mN m-1 reduction in surface tension. The effects of different pH (4.2-9.2), salinity concentrations (1%-15%), and temperatures (25-50) in biosurfactant production of isolated strains were evaluated. One of the strains (Bacillus sp. NO.4) showed a high salt tolerance and a successful production of biosurfactant in a vast pH range. Its maximum biomass production (about 3.1 g L-1 dry weight) was achieved after 60 hours of growth. The surface tension of the culture broth dropped rapidly after inoculation and reached its lowest value (36 mN m-1) during the exponential phase after about 36-48 hours of growth. The study of the GC graphs showed that higher aliphatic reduction occurred in fractions with C14 to C24 hydrocarbons. The depicted results of the HPLC graphs indicated a 100% degradation of chrysene and fluorine. In this study, we demonstrated the useful capacities of the isolates in removing oil pollutants and their application in MEOR in vitro.
机译：本研究调查了从伊朗油井中分离并鉴定出产生生物表面活性剂的细菌。使用溶血和乳化测试评估并确认了细菌分离物的生物表面活性剂生产。使用GC和HPLC分析研究了原油的生物降解。总共分离出45个菌株。这些应变显示表面张力降低不到40 mN m-1。评估了不同pH（4.2-9.2），盐度浓度（1％-15％）和温度（25-50）在分离菌株的生物表面活性剂生产中的影响。一种菌株（芽孢杆菌NO.4）显示出高的耐盐性，并在宽pH范围内成功生产了生物表面活性剂。生长60小时后，达到了最大的生物量生产（约3.1 g L-1干重）。接种后培养液的表面张力迅速下降，并在生长约36-48小时后在指数期达到最低值（36 mN m-1）。 GC图的研究表明，在C14至C24烃馏分中，脂肪族化合物的还原率更高。 HPLC图的描绘结果表明，铬和氟的降解率为100％。在这项研究中，我们证明了分离株在去除油污污染物方面的有用能力及其在体外MEOR中的应用。

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