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Biodegradation of malathion by serratia marcescens isolated from Arvandkenar region, Iran

机译:从伊朗阿凡德纳尔地区分离的粘质沙雷氏菌对马拉硫磷的生物降解作用

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The global use of pesticides has resulted in the contamination of various ecosystems worldwide. The impact of these pesticides can be reduced through bioremediation. The factors that influence the biodegradation rate include the isolation of efficient bacteria for use in remediation and the determination of optimal biodegradation conditions. In this study, malathion degrading bacteria were isolated from agricultural soil samples taken from the Arvandkenar region in Iran. To optimize the biodegradation of malathion by an isolated strain, the effect of four parameters (temperature, salinity, NH4Cl and K2HPO4) was evaluated while considering protein concentrations at different times. The malathion remaining in the media was measured using the gas chromatography method. A gram-negative bacterium strain BNA1 with malathion biodegrading ability was isolated from the soil sample which showed a 99% similarity to Serratia marcescens. The optimum biodegradation condition occurred at a temperature = 30 ?C, salinity = 0 %, NH4Cl = 0.25 g/L and K2HPO4 = 0.25 g/L. A biodegradation efficiency of 65% was obtained under the above-mentioned condition. The results of this study revealed the significant capability of BNA1 in the biodegradation of malathion. Therefore, the use of an isolated strain may be considered as an important tool in the bioremediation of pesticide-contaminated soil.
机译:农药的全球使用导致全世界各种生态系统的污染。这些农药的影响可以通过生物修复来减少。影响生物降解速率的因素包括有效修复细菌的分离和最佳生物降解条件的确定。在这项研究中,从伊朗Arvandkenar地区采集的农业土壤样品中分离了马拉硫磷降解细菌。为了优化分离菌株对马拉硫磷的生物降解,评估了四个参数(温度,盐度,NH4Cl和K2HPO4)的影响,同时考虑了不同时间的蛋白质浓度。使用气相色谱法测量残留在培养基中的马拉硫磷。从土壤样品中分离出具有马拉硫磷生物降解能力的革兰氏阴性细菌菌株BNA1,该菌株与粘质沙雷氏菌有99%的相似性。最佳的生物降解条件发生在温度= 30°C,盐度= 0%,NH4Cl = 0.25 g / L和K2HPO4 = 0.25 g / L的条件下。在上述条件下获得65%的生物降解效率。这项研究的结果表明BNA1在马拉硫磷的生物降解中具有显着的能力。因此,分离菌株的使用可以被认为是对农药污染土壤进行生物修复的重要工具。

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