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首页> 外文期刊>African Journal of Biotechnology >Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia
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Comparative effectiveness of inter-simple sequence repeat and randomly amplified polymorphic DNA markers to study genetic diversity of Indian Garcinia

机译：简单序列重复和随机扩增的多态性DNA标记对印度藤本植物遗传多样性研究的比较有效性

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摘要

A study to compare the effectiveness of inter-simple sequence repeats (ISSR) and randomly amplified polymorphic DNA (RAPD) profiling was carried out with a total of 65 DNA samples using 12 species of Indian Garcinia. ISSR and RAPD profiling were performed with 19 and 12 primers, respectively. ISSR markers generated a total number of 156 bands with 92 polymorphic bands, while RAPD markers produced a total of 134 bands with 80 polymorphic bands. Percentage of polymorphic loci in RAPD profiling was 60.4% while in ISSR profiling, it was 59.3%. Heterogeneity index was similar for the markers, 0.86 for ISSR and 0.89 for RAPD, indicating that both the marker systems are effective in determining polymorphism in Garcinia. ISSR markers showed clear distinction among the species whereas RAPD markers showed segregation based on geographical location as well as species based.
机译：使用12种印度藤本植物对总共65个DNA样本进行了比较简单序列间重复（ISSR）和随机扩增多态性DNA（RAPD）分析的有效性的研究。用19和12个引物分别进行ISSR和RAPD分析。 ISSR标记产生了总共156条带，其中有92个多态性条带，而RAPD标记产生了总共134条带，其中有80个多态性条带。 RAPD分析中多态性位点的百分比为60.4％，而ISSR分析中为59.3％。标记的异质性指数相似，ISSR的异质性指数为0.86，RAPD的异质性指数为0.89，表明两种标记系统均可有效测定藤黄的多态性。 ISSR标记显示了物种之间的明显区别，而RAPD标记显示了基于地理位置和基于物种的分离。

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