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首页> 外文期刊>African Journal of Biotechnology >Characterization of Burkholderia cepacia genomovar I as a potential biocontrol agent of Ganoderma boninense in oil palm
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Characterization of Burkholderia cepacia genomovar I as a potential biocontrol agent of Ganoderma boninense in oil palm

机译：表征伯克霍尔德菌洋葱伯克霍尔德菌I作为油棕中灵芝boninense的潜在生物防治剂

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Burkholderia?is an important bacterial genus containing several species of ecological, biotechnological and pathological interest. Bacterial isolate can be gotten from soil, water, plants and even clinics. With their taxonomy undergoing constant revision and the phenotypic similarity of several species, correct identification ofBurkholderia?is difficult.?Burkholderia cepacia complex (Bcc) consists of nine discrete genomic species and a genetic scheme based on the?recA?gene has greatly enhanced the identification of?B. cepacia?complex species.?The objectives of this study were to identify?Burkholderia strain?UPM B3 which was isolated from oil palm roots to the species level based on Biolog? Identification System, and to carry out DNA fingerprinting for strain differentiation as well as differentiate between pathogenic and non-pathogenic human forms. Antagonistic activity of UPM B3 against?Ganoderma boninense?was also evaluated by using dual culture and poison food tests. Genotype characterization was carried out by amplification of the?recAgene using specific primers, purified using QIA Quick polymerase chain reaction (PCR) purification kit and sequenced. Multiple sequence alignments were performed on closely related sequence accessions using CLUSTAL W software. Result of nucleotide sequencing followed by phylogenetic analysis of the?recAfragments differentiated both putative and known?Burkholderia?species and all members of the?B. cepacia?complex. Genomovar analysis confirmed that UPM B3, isolated from oil palm roots belongs to genomovar I and has antagonistic activity against?G. boninense?based on?in vitro?dual culture and poison food tests. From the phylogenetic tree, UPM B3 is a specific strain within?B. cepacia?complex species that belong to genovomar I which is associated with strains nonpathogenic to humans. Thus,?B. cepacia?strain UPM B3 has the potential to be used against?G.boninense, the causal pathogen of basal stem rot (BSR) in oil palm.
机译：伯克霍尔德氏菌是一种重要的细菌属，包含多种具有生态，生物技术和病理学意义的物种。细菌分离物可以从土壤，水，植物甚至诊所获得。随着分类学的不断修订和几种物种的表型相似性，对伯克霍尔德菌的正确鉴定是困难的。伯克霍尔德菌洋葱复合体（Bcc）由9个离散的基因组物种组成，基于recA基因的遗传方案大大增强了鉴定。的？这项研究的目的是鉴定根据油菜属植物根系分离出的伯克霍尔德菌菌株UPM B3。鉴定系统，并进行DNA指纹识别以区分菌株，以及区分致病性和非致病性人类形式。还通过双重培养和有毒食品试验评价了UPM B3对“灵芝博纳氏菌”的拮抗活性。通过使用特异性引物扩增ΔrecA基因，使用QIA Quick聚合酶链反应（PCR）纯化试剂盒纯化并测序，进行基因型表征。使用CLUSTAL W软件对紧密相关的序列登录进行了多个序列比对。核苷酸测序的结果，然后对recAfragments进行系统发育分析，区分了推定的和已知的伯克霍尔德氏菌物种和所有B成员。 cepacia？complex。 Genomovar分析证实，从油棕根中分离出的UPM B3属于Genomovar I，对βG具有拮抗活性。基于体外双重培养和有毒食品测试的boninense。从系统发育树来看，UPM B3是？B中的特定菌株。属于Genovomar I的头孢菌素复合体物种，与人类非致病性菌株有关。因此，？洋葱头孢菌素UPM B3有潜力用于对抗G.boninense，后者是油棕中基底茎腐病（BSR）的致病菌。

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