首页> 外文期刊>African Journal of Biotechnology >Genotypic frequency of calpastatin gene in lori sheep by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method
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Genotypic frequency of calpastatin gene in lori sheep by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method

机译:聚合酶链反应-限制性片段长度多态性(PCR-RFLP)方法检测洛里绵羊钙蛋白酶抑制素基因的基因型频率

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Calpastatin is a natural occurring inhibitor of calpastatin (CAST) and consequently the balance of calpain-calpastatin activity in muscles is believed to dictate the rate of tenderization in post-mortem meat. Genomic DNA was extracted from 100 sheep blood sample. Polymerase chain reaction was performed to amplify a 622 bp fragment of this gene. Restriction reaction of polymerase chain reaction (PCR) products was done using MspI?enzyme. The MspI digestion of the PCR products produced digestion fragments of 336 and 286 bp. The results show that in the population, genotypes AA, AB and BB, respectively, had frequencies 32.2, 63.2 and 4.6, and that this locus was not at Hardy?-?Weinberg equilibrium in the lori sheep strain (P<0.05).
机译:钙蛋白酶抑制剂是钙蛋白酶抑制剂(CAST)的天然抑制剂,因此,人们认为肌肉中钙蛋白酶钙蛋白酶抑制剂活性的平衡决定了宰后肉的嫩化率。从100只绵羊血液样本中提取基因组DNA。进行聚合酶链反应以扩增该基因的622bp片段。聚合酶链反应(PCR)产物的限制性反应使用MspI酶完成。 PCR产物的MspI消化产生了336和286bp的消化片段。结果表明,在种群中,AA,AB和BB基因型分别具有32.2、63.2和4.6的频率,并且该基因座不在洛里绵羊品系中的Hardy?-?Weinberg平衡处(P <0.05)。

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