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首页> 外文期刊>African Journal of Biotechnology >Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens
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Antimicrobial activity of metabolites extracted from Zanthoxylum gilletii, Markhamia lutea and their endophytic fungi against common bean bacterial pathogens

机译：从花椒，Markhamia lutea及其内生真菌中提取的代谢物对普通豆类细菌病原菌的抗菌活性

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Antibacterial activity of extracts of Zanthoxylum gilletii, Markhamia lutea and their fungal endophytes were evaluated against bacterial pathogens of common bean: Xanthomonus axonopodis pv. phaseoli and Pseudomonus syringae pv. phaseolicola. The leaves of both plants were dried under shade, ground to fine powder and extracted using methanol. The methanol extracts were fractionated sequentially using ethylacetate and hexane to produce various fractions. Endophytic fungi were isolated from fresh leaves and identified by ITS-rDNA sequence analysis. Antibacterial screening of the fungal endophytes was done by dual culture assay. The most active endophytic fungi were fermented on rice media and extracted using methanol. Pure compounds were analyzed by a combination of mass spectrometry and spectroscopic techniques which included 1D and 2D NMR. Antibacterial activity of all the extracts was determined by disc agar diffusion assay against the test organisms. Twenty-four (24) fungal endophytes were isolated which included: Fusarium, Chaetomium, Scopulariopsis and Trametes. Endophytic Fusarium solani was the most active against X. axonopodis pv. phaseoli (20.3 mm inhibition zone) and P. syringae pv. phaseolicola (18.6 mm inhibition zone). The plant extracts were active against X. axonopodis pv. phaseoli with an inhibition zone ranging between 8-12 mm except the methanol extract from Z. gilletii which did not show any activity. The endophytic extracts were active against both test organisms with a zone of inhibition ranging from 9.3-14 mm. Phenolic compounds present in Fusarium species may have contributed to the antibacterial activity of this strain against the test organisms.
机译：评价了花椒（Zanthoxylum gilletii），黄花Markhamia lutea及其真菌内生菌的提取物对普通豆类细菌病原体（Xanthomonus axonopodis pv）的抗菌活性。菜豆和丁香假单胞菌。菜豆。将两种植物的叶子在阴凉处干燥，研磨成细粉并用甲醇萃取。依次使用乙酸乙酯和己烷将甲醇提取物分级分离以产生各种馏分。从新鲜叶子中分离出内生真菌，并通过ITS-rDNA序列分析进行鉴定。通过双重培养测定法对真菌内生菌进行了抗菌筛选。在水稻培养基上发酵最活跃的内生真菌，并用甲醇提取。通过质谱和光谱技术（包括1D和2D NMR）的组合分析纯化合物。通过盘状琼脂扩散测定法测定所有提取物对测试生物的抗菌活性。分离出二十四（24）个真菌内生菌，包括：镰刀菌属，毛毛虫属，鞘翅目和孔形菌。内生镰刀菌对X. axonopodis pv最活跃。菜豆（20.3毫米抑制区）和丁香假单胞菌pv。菜豆（禁区18.6 mm）。该植物提取物具有抗轴突X. pv的活性。除来自吉尔茨氏菌的甲醇提取物没有任何活性外，抑制区的范围在8-12毫米之间。内生提取物对两种测试生物均具有抑制范围为9.3-14 mm的活性。镰刀菌属中存在的酚类化合物可能有助于该菌株对测试生物的抗菌活性。

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