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首页> 外文期刊>African Journal of Biochemistry Research >Extraction, optimization and characterization of crude polysaccharides from Artemesia Mongolica
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Extraction, optimization and characterization of crude polysaccharides from Artemesia Mongolica

机译：蒙古蒿（Artemesia Mongolica）粗多糖的提取，优化和表征

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In this study, the extraction conditions of polysaccharides of Artemisia mongolia (PAM) were optimized and their molecular weight, weight distribution, composition and antioxidant activities of the polysaccharides investigated. The degree of polymerization of fructan-oligosaccharides 2-8 was separated by thin layer chromatography (TLC) with good results. Polysaccharides were extracted from the leaves of A. mongolia and the effects of the extraction parameters (extraction temperature, extraction time and water to raw material ratio) were optimized under optimal extraction combinations. Based on the study, the maximum yield of polysaccharides (17.38%) was obtained at an extraction temperature of 76.90°C, extraction time of 1.33 h and water-to-raw material ratio of 4.00 ml/mg. The yield of polysaccharides was largely dependent on the extraction parameters. Four isolated fractions of the polysaccharides were further characterized using high-performance liquid chromatography. Using TLC analysis, the degree of polymerization of fructan-oligosaccharides 2-8 was successfully separated. High A. mongolia antioxidant polysaccharide with antioxidant activity of 351.3 sc/g was obtained at optimized incubation temperature of 70.37°C, extraction time of 1.45 h and water-to-raw material ratio of 2 ml/mg.
机译：本研究对青蒿多糖的提取条件进行了优化，并对其分子量，重量分布，组成和抗氧化活性进行了研究。通过薄层色谱法（TLC）分离出果聚糖-低聚糖2-8的聚合度，结果良好。从蒙古曲霉的叶片中提取多糖，并在最佳提取组合条件下优化了提取参数（提取温度，提取时间和水与原料比）的影响。根据该研究，在76.90°C的萃取温度，1.33 h的萃取时间和4.00 ml / mg的水/原料比下，多糖的最大产量（17.38％）获得了。多糖的产量主要取决于提取参数。使用高效液相色谱进一步表征了四个分离的多糖级分。使用TLC分析，成功分离了果聚糖-低聚糖2-8的聚合度。在70.37°C的最佳培养温度，1.45 h的提取时间和2 ml / mg的水/原料比下，获得了具有351.3 sc / g抗氧化活性的高蒙古抗氧化多糖。

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