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首页> 外文期刊>RSC Advances >Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa
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Optimization of the extraction, preliminary characterization, and anti-inflammatory activity of crude polysaccharides from the stems of Trapa quadrispinosa

机译：菱Tra茎粗多糖的提取，初步表征和抗炎活性的优化

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摘要

A novel method was developed using pressurized-assisted extraction (PAE) to efficiently extract crude Trapa quadrispinosa polysaccharides (TQCPS) from plant stems, and the extraction process was optimized using response surface methodology (RSM). At a water-to-material fixed ratio of 30 mL g ~(?1) , the highest yield of 3.72 ± 0.13% was obtained under the optimum conditions of extraction time of 32 min, extraction temperature at 47 °C, and extraction pressure at 1.87 Mpa, which were in agreement with the predicted value of 3.683%. Compared with conventional hot water extraction (HWE), the PAE method remarkably enhanced the extraction yield with the further advantages of short extraction time and low extraction temperature. The preliminary characteristics of TQCPS were analyzed through UV-vis, FT-IR, and chemical composition analysis. In subsequent anti-inflammatory studies, when RAW 264.7 mouse macrophage cells were treated with TQCPS, satisfactory anti-inflammatory activity was observed, and TQCPS significantly suppressed the release of nitric oxide (NO), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) and synchronously restrained the expression levels of inducible nitric oxide synthase (iNOS), TNF-α, and IL-6 mRNA induced by lipopolysaccharide (LPS) in a dose-dependent manner. These results indicate that PAE is a technology that can be used for efficient extraction of polysaccharides from medicinal plants, and TQCPS can be explored as a potential anti-inflammatory agent in medicine.
机译：开发了一种利用加压辅助提取（PAE）从植物茎中有效提取粗四叶多糖（TQCPS）的新方法，并使用响应面法（RSM）优化了提取工艺。在30 mL g〜（？1）的水-材料固定比下，在32分钟的萃取时间，47°C的萃取温度和萃取压力的最佳条件下，最高收率为3.72±0.13％。压力为1.87 Mpa，与3.683％的预测值相符。与传统的热水萃取（HWE）相比，PAE方法显着提高了萃取率，并且萃取时间短，萃取温度低。通过UV-vis，FT-IR和化学成分分析来分析TQCPS的初步特征。在随后的抗炎研究中，当用TQCPS处理RAW 264.7小鼠巨噬细胞时，观察到令人满意的抗炎活性，TQCPS显着抑制了一氧化氮（NO），肿瘤坏死因子-α（TNF-α）的释放，白介素6（IL-6）和脂多糖（LPS）诱导的诱导型一氧化氮合酶（iNOS），TNF-α和IL-6 mRNA的表达水平呈剂量依赖性。这些结果表明，PAE是一种可用于从药用植物中有效提取多糖的技术，TQCPS可作为一种潜在的抗炎药进行研究。

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