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首页> 外文期刊>African Journal of Biotechnology >Psychrotrophic bacteria isolated from -20C freezer
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Psychrotrophic bacteria isolated from -20C freezer

机译：从-20C冰箱中分离出的精神营养菌

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Three psychrotrophic bacteria, morpho-physiologically, identified as?Bacillus subtilisMRLBA7,?Bacillus licheniformis?MRLBA8 and?Bacillus megaterium?MRLBA9 were isolated from -20°C freezer of the Microbiology Research Laboratory (MRL), Quaid-i-Azam University, Islamabad, Pakistan. These strains were able to grow aerobically at 6°C but not at 40°C except MRLBA8 that could grow at 48°C. None of the isolates showed inhibition of growth in the presence of glycerol. Isolate MRLBA7, bearing central spore, grew in the presence of 30% glycerol at 0°C after 48 h of incubation and showed maximum growth without glycerol at 25°C after 24 h. Isolate MRLBA8 showed growth in the presence of 50% glycerol at 4°C after 72 h of incubation and maximum growth was observed at 20°C in the absence of glycerol. Isolate MRLBA9 showed growth at 6°C in the presence of 40% glycerol after 48 h of incubation and maximum growth was observed at 25°C in the absence of glycerol. Isolates were susceptible to antibiotics except?Bacillus subtilis?MRLBA7 that exhibited antibiotic resistance against penicillin and fosphomycin,?Bacillus licheniformis?MRLBA8 against aztreonam and fosphomycin, and?Bacillus megaterium?MRLBA9 against vancomycin and penicillin. The growth profile and biochemical characteristics of all the isolates were rather similar to that of mesophilic counterparts except adaptation to low temperature. These strains could be used as model microbial strains for characterization of food contaminants in freezers, to understand the mechanism of antibiotic resistance induced at low temperature and as a source of psychrotrophic enzymes.
机译：从-20°C冷冻机中分离出3种精神形态细菌，在形态生理上被鉴定为枯草芽孢杆菌MRLBA7，地衣芽孢杆菌MRLBA8和巨大芽孢杆菌MRLBA9。 ，巴基斯坦。这些菌株能够在6°C有氧生长，但在40°C不能生长，但MRLBA8可以在48°C生长。在甘油的存在下，没有分离物显示出生长抑制作用。孵育48小时后，带有中央孢子的分离物MRLBA7在30％的甘油存在下于0°C下生长，并在25°C下在无甘油的条件下显示出最大的生长。孵育72小时后，分离物MRLBA8在4％的50％甘油存在下显示生长，在不存在甘油的情况下在20°C观察到最大生长。孵育48小时后，分离株MRLBA9在40％甘油存在下于6°C下生长，在不存在甘油的情况下在25°C下观察到最大生长。分离株对抗生素敏感，除了对青霉素和磷霉素表现出抗药性的枯草芽孢杆菌MRLBA7，对氨曲南和磷霉素表现出地衣芽孢杆菌MRLBA8，对万古霉素和青霉素表现出巨大芽孢杆菌MRLBA9。除适应低温外，所有分离株的生长概况和生化特性均与嗜温菌相似。这些菌株可以用作模型微生物菌株，用于表征冰柜中的食物污染物，以了解低温诱导的抗生素抗性机制，并作为精神营养酶的来源。

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