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首页> 外文期刊>African Journal of Biotechnology >Simple and efficient methods for isolation and activity measurement of the recombinant hirudin variant 3 from Bacillus subtilis
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Simple and efficient methods for isolation and activity measurement of the recombinant hirudin variant 3 from Bacillus subtilis

机译：枯草芽孢杆菌重组水rud素变体3分离和活性测定的简单有效方法

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摘要

A simple purification approach of the recombinant hirudin variant 3 from the?Bacillus subtilis?was established, by which the hirudin could be purified to the purity of 95% through one-step chromatography with the total recovery rate of 83.9%. A modified Markwardt thrombin titration method for measuring hirudin activity was also set up.Briefly, a series of concentrations of thrombin was prepared and titrated to hirudin sample, respectively and the anti-thrombin activity-range of hirudin was narrowed down by several thrombin solutions at high or low concentration and the optimum group of thrombin concentrations was determined for titration of the hirudin sample. In this modified method, the hirudin activity was determined more accurately, concisely and promptly than the classic Markwardt method.
机译：建立了枯草芽孢杆菌重组水？素变体3的简单纯化方法，可通过一步色谱法将水in素纯化至95％的纯度，总回收率为83.9％。还建立了一种改良的Markwardt凝血酶滴定法来测定水activity素活性。简要地制备了一系列浓度的凝血酶，并将其滴定至水rud素样品中，并在几种凝血酶溶液中将水rud素的抗凝血酶活性范围缩小了。确定水or素样品的高低浓度和最佳凝血酶浓度组。在这种改进的方法中，水rud素活性的测定比经典的Markwardt方法更准确，简洁和迅速。

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