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Cat epididymal semen cryopreserved with and without vitamin E: effect on sperm parameters and lipid peroxidation

机译：冷冻含和不含维生素E的猫附睾精液：对精子参数和脂质过氧化的影响

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The aims of this study were to investigate: 1) if the addition of α-tocopherol (vitamin E) in three concentrations (0.3, 0.6 and 0.9 mM) is able to preserve spermatozoa integrity after thawing and 2) the effect of a-tocopherol supplementation on lipid peroxidation. Fifty four domestic cats were used in this study constituting 18 pools (3 cats per pool). Each pool was submitted at four experimental groups: group 0 (control) – epididymal sperm were frozen with a commercial Botucrio ? extender; group 0.3, group 0.6 and group 0.9 – the extender was supplemented with 0.3, 0.6 and 0.9 mM of a-tocopherol, respectively. Each semen sample was evaluated for motility, progressive forward motility, morphology, sperm viability (plasma membrane integrity-PMI), hypo-osmotic swelling test (HOST), before and after thawing. The evaluation of lipid peroxidation reaction by Thiobarbituric Acid Reactive Substances (TBARS) test was performed on thawed semen only. Results demonstrated that there was no significant difference between control and the three a-tocopherol groups with regards to motility and progressive motility after thawing (P 0.05). As expected, in fresh samples viability was significantly higher than in all the cryopreserved groups in which there was no positive influence of any of the a-tocopherol concentration used. Lipid peroxidation was higher in the supplemented groups 0.6 and 0.9 mM of a-tocopherol than in control and in 0.3 mM group. In conclusion, the addition of a-tocopherol to the commercial extender had no positive influence on reduction of lipid peroxidation. This topic deserves further investigations to better understand the effect of cryopreservation procedures on epididymal spermatozoa and to establish adequate strategies to counteract sperm cryodamages.
机译：这项研究的目的是研究：1）在三种浓度（0.3、0.6和0.9 mM）中添加α-生育酚（维生素E）是否能够在融化后保持精子的完整性，以及2）α-生育酚的作用补充脂质过氧化。在这项研究中，使用了54只家猫，共构成18个池（每池3只猫）。每个库均分为四个实验组：第0组（对照组）–用市售Botucrio？冷冻附睾精子？补充剂0.3、0.6和0.9组–补充剂分别补充了0.3、0.6和0.9 mM的α-生育酚。在解冻之前和之后，评估每个精液样品的运动性，进行性向前运动性，形态，精子生存力（质膜完整性-PMI），低渗透溶胀试验（HOST）。仅通过解冻精液通过硫代巴比妥酸反应性物质（TBARS）测试评估脂质过氧化反应。结果表明，对照组和三个α-生育酚组之间在融化后的运动性和进行性运动方面无显着差异（P> 0.05）。如预期的那样，在新鲜样品中，活力显着高于所有冷冻保存的组，在冷冻保存的组中，使用的任何α-生育酚浓度均无积极影响。补充组0.6和0.9 mM的α-生育酚的脂质过氧化作用高于对照组和0.3 mM组。总之，向商业增量剂中添加α-生育酚对降低脂质过氧化没有积极影响。该主题值得进一步研究，以更好地了解冷冻保存程序对附睾精子的影响，并建立应对精子冷冻损害的适当策略。

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