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首页> 外文期刊>Asian Journal of Pharmaceutical and Clinical Research >EVALUATION OF THE CYTOCHROME P450 INHIBITORY EFFECT OF THYME OLEORESIN FROM THYMUS VULGARIS L. - AN IN VITRO STUDYEVALUATION OF THE CYTOCHROME P450 INHIBITORY EFFECT OF THYME OLEORESIN FROM THYMUS VULGARIS L. - AN IN VITRO STUDY
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EVALUATION OF THE CYTOCHROME P450 INHIBITORY EFFECT OF THYME OLEORESIN FROM THYMUS VULGARIS L. - AN IN VITRO STUDYEVALUATION OF THE CYTOCHROME P450 INHIBITORY EFFECT OF THYME OLEORESIN FROM THYMUS VULGARIS L. - AN IN VITRO STUDY

机译：寻常百里香中百里香精油的细胞色素P450抑制作用的评价-体外研究寻常百里香中百里香精油的细胞色素P450抑制作用的评价-体外研究。

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Objective: The objective of this study was to evaluate the effect of thyme oleoresin on cytochrome P450 (CYP3A4) enzyme. Materials and Methods: The different concentrations of thyme oleoresin (5–100 μg/ml) were examined for its inhibitory property toward cytochrome P450 isoform (CYP3A4). Thyme oleoresin, potassium phosphate buffer, CYP450 reagent, and substrate 7-Benzyloxy-4-trifluoromethylcoumarin were added to a 96-well plate. The mixtures were preincubated for 20 min at room temperature. The fluorescent intensities of the products were measured by PerkinElmer Enspire fluorescence reader using an excitation and emission wavelength of 405 nm and 460 nm, respectively. Values were expressed as mean ± standard error mean (n=3). IC50 was calculated by plotting concentrations of thyme against the corresponding percent inhibition. Results: All the tested concentrations of thyme showed inhibitory effect against CYP3A4 in a dose-dependent manner. At 5 μg/ml, it showed a percentage inhibition of 1.82±0.61, whereas 100 μg/ml showed 66.05±0.16. The IC50 value of thyme for CYP3A4 inhibitory activity was found to be 39.14 μg/ml. Conclusion: This study prove the inhibitory effect of thyme oleoresin on cytochrome P450. The inhibitory effect of thyme oleoresin indicates the possibilities of herb-drug interaction if this extract is co-administered with prescribed drugs that are metabolized by CYP3A4.
机译：目的：本研究的目的是评估百里香油树脂对细胞色素P450（CYP3A4）酶的影响。材料和方法：检查了不同浓度的百里香油树脂（5-100μg/ ml）对细胞色素P450同工型（CYP3A4）的抑制作用。将百里香脂树脂，磷酸钾缓冲液，CYP450试剂和底物7-苄氧基-4-三氟甲基香豆素添加到96孔板中。将混合物在室温下预孵育20分钟。通过PerkinElmer Enspire荧光读取器分别使用405 nm和460 nm的激发和发射波长测量产物的荧光强度。值表示为平均值±标准误差平均值（n = 3）。通过将百里香浓度与相应的抑制百分数作图来计算IC50。结果：所有测试浓度的百里香均显示出对CYP3A4的抑制作用，呈剂量依赖性。在5μg/ ml时，其抑制百分比为1.82±0.61，而100μg/ ml时显示为66.05±0.16。发现百里香对CYP3A4抑制活性的IC50值为39​​.14μg/ ml。结论：本研究证明了百里香油树脂对细胞色素P450的抑制作用。百里香油树脂的抑制作用表明，如果该提取物与经CYP3A4代谢的处方药共同给药，则可能具有药草相互作用。

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