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Determination of Ascorbic Acid by Electrochemical Techniques and other Methods

机译:电化学技术和其他方法测定抗坏血酸

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Ascorbic acid (AA) is a water-soluble vitamin essential in the human nutrition, an antioxidant, ascavenger of free radicals in biological systems and a cofactor of several enzymes. For the precisedetection of AA concentration in biological samples there is important the option of the determinationmethod. To the most common and exact methods for AA evaluation belong HPLC techniques with UVand electrochemical detection, and electrochemical methods (EM). EM are simple, sensitive, andmoderate methods. For AA detection different types of electrodes are suitable, lately there are usedespecially various chemically modified electrodes with applied polymer films. Popular and profitablefor accurate AA determination there are electrochemical biosensors and voltammetry that is reputabledue to the low detection limits of AA.
机译:抗坏血酸(AA)是人类营养中必不可少的水溶性维生素,抗氧化剂,生物系统中自由基的清除剂以及多种酶的辅助因子。为了精确检测生物样品中的AA浓度,重要的是选择测定方法。最常用和最准确的AA评估方法是采用UV和电化学检测的HPLC技术以及电化学方法(EM)。 EM是简单,敏感和适度的方法。对于AA检测,不同类型的电极是合适的,最近,尤其是使用了具有施加的聚合物膜的各种化学改性的电极。对于准确的AA测定而言,流行且有利可图的是电化学生物传感器和伏安法,由于AA的检测限较低,因此享有盛誉。

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