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Quantitative Analysis of Caffeine Content from Different Tea Growing Regions of Rwanda

机译:卢旺达不同茶区的咖啡因含量的定量分析

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In recent times, tea becomes highly known owing to its pharmacological potential properties such as antitumor, anti-oxidative, and anti-carcinogen activities. Especially, the quality of tea is assessed based on its caffeine content. Most people drink tea with the purpose of getting some caffeine amount in their body to diminish the increased cardiovascular disease, some chronic diseases, and cancer risks. The main study's objective was to determine the caffeine content in tea samples from different tea growing regions of Rwanda in order to identify the region that has tea with the highest amount of caffeine. This may help in classifying the tea regions and tea quality in Rwanda according to their amount of caffeine. In this study, eleven samples were obtained from National Agriculture Export development Board (NAEB). Extraction of the caffeine was done using hot water. Quantification of caffeine from tea samples was accomplished by using high-pressure liquid chromatography (HPLC); the mixture of methanol (20%), deionized water (79%) and acetic acid (1%) [(20:79:1)] was used as HPLC's mobile phase. The tea sample grown at Rubaya tea growing region was obtained to have the highest caffeine quantity of 802.927±40.04 ppm. This was followed by tea grown at Nyabihu which contained caffeine quantity of 798.937±19.74 ppm. The least caffeine quantity was obtained in tea sample taken from Shagasha region with caffeine quantity of 476.128±97.05. Our results indicated that the caffeine content from tea growing regions in Rwanda had a range from 476.128±97.05 to 802.927±40.04 ppm, also had different tea quality.
机译:近年来,由于其具有抗肿瘤,抗氧化和抗致癌活性等药理潜力,茶已广为人知。尤其是,茶的质量是根据其咖啡因含量进行评估的。大多数人喝茶的目的是在体内摄取一定量的咖啡因,以减少心血管疾病,某些慢性疾病和癌症的风险。主要研究的目的是确定卢旺达不同产茶区茶样品中的咖啡因含量,以鉴定含咖啡因含量最高的茶的地区。这可能有助于根据卢旺达的咖啡因含量对茶叶区域和茶叶质量进行分类。在这项研究中,从国家农业出口发展委员会(NAEB)获得了11个样本。咖啡因的提取使用热水进行。通过使用高压液相色谱(HPLC)对茶叶样品中的咖啡因进行定量。甲醇(20%),去离子水(79%)和乙酸(1%)[(20:79:1)]的混合物用作HPLC的流动相。获得在Rubaya茶产区种植的茶样品,咖啡因含量最高,为802.927±40.04 ppm。随后在Nyabihu种植的茶中咖啡因含量为798.937±19.74 ppm。从Shagasha地区采集的茶样品中咖啡因含量最低,咖啡因含量为476.128±97.05。我们的结果表明,卢旺达产茶区的咖啡因含量范围为476.128±97.05 ppm至802.927±40.04 ppm,并且茶叶质量也有所不同。

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