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Isolation and Characterization of a Bacterial Strain Capable of Efficient Berberine Degradation

机译:能够有效降解小grad碱的细菌菌株的分离和鉴定

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Background: Berberine (BBR) is a pharmaceutical chemical with a broad antibacterial spectrum, and its biological treatment has been of research and practical interest. In this study, a pure bacterial strain B16 was isolated from the activated sludge in a pharmaceutical wastewater treatment plant. The aim of the study is to characterize the properties of the strain B16, especially its BBR degradation capability. Methods: The identification of strain B16 was conducted by visual observation, as well as biochemical and phylogenetic analysis. The degradation kinetics of strain B16 was tentatively described by Haldane model. Results: The strain B16 was 100% determined as a Sphingopyxis sp. The kinetic parameters of BBR degradation by strain B16 were as follows: Vmax 54.73 ± 5.54 mg (g MLSS · h) ?1 , Km 66.68 ± 8.95 mg L ?1 , and Ki 43.16 ± 5.92 mg L ?1 , with an R 2 of 0.996. Stain B16 exhibited considerable capability of BBR degradation. BBR of initial concentration 40 mg L ?1 could be completely degraded in 48 h under optimal conditions. Conclusions: strain B16 was the first pure culture found with the ability to totally mineralize BBR, indicating the potential of B16 application in real industrial processes.
机译:背景:小Ber碱(BBR)是一种具有广泛抗菌谱的医药化学品,其生物治疗已引起研究和实际兴趣。在这项研究中,从制药废水处理厂的活性污泥中分离出纯细菌菌株B16。研究的目的是表征菌株B16的特性,尤其是其BBR降解能力。方法:通过肉眼观察以及生化和系统发育分析鉴定B16菌株。用Haldane模型初步描述了菌株B16的降解动力学。结果:菌株B16被100%确定为Sphingopyxis sp。菌株B16降解BBR的动力学参数如下:Vmax 54.73±5.54 mg(g MLSS·h)?1,Km 66.68±8.95 mg L?1和Ki 43.16±5.92 mg L?1,R 2为0.996。染色剂B16表现出相当的BBR降解能力。在最佳条件下,初始浓度为40 mg L?1的BBR在48小时内可以完全降解。结论:B16菌株是发现的第一个具有完全矿化BBR能力的纯培养物,表明B16在实际工业过程中的应用潜力。

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