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首页> 外文期刊>International Journal of Poultry Sciences >Application of Coliphage Lysate: A Preliminary Trial to Treat an Experimental Escherichia coli Infection in Broiler Chicken
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Application of Coliphage Lysate: A Preliminary Trial to Treat an Experimental Escherichia coli Infection in Broiler Chicken

机译：鹅卵石裂解液的应用：治疗肉鸡实验性大肠杆菌感染的初步试验

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Pathogenic E. coli was isolated from the birds suffering from colibacillosis showing characteristic lesions. Two types of coliphages T1 and T4 were isolated from the sewage water collected from different areas of Faisalabad, Pakistan. These phages were separately tested for in vitro lysis of their host cell on tryptone agar plates, with equal (1:1)) concentration (107 CFU and 107 PFU) of E. coli and phages, which resulted in complete absence of colonies of bacteria after 10 minutes. When phage concentration was reduced (1:? and 1::?), colonies continued to appear even after 10 minutes. Later these phages were mixed and evaluated through in vivo trials in broiler chickens of two weeks age. A total of 100 broilers were divided into four groups (A, B, C and D) having 25 birds in each group. First three groups were administered with equal (1:1) concentration of phages as well as E. coli (107/ml) through oral, intraperitoneal and intramuscular routes. The control group (D) was further subdivided into two groups of D1 and D2, both of which were deprived of phages and E. coli respectively. The 13 birds of group D2 that were given only the phages did show neither the signs of disease nor mortality. The results indicated a good protection level using coliphages when compared with the control group. It was concluded that phages may be used effectively as good alternatives of those antibiotics to which bacteria have become resistant.
机译：从患有大肠埃希氏菌病的鸟类中分离出致病性大肠杆菌，表现出特征性病变。从巴基斯坦费萨拉巴德不同地区收集的污水中分离出两种类型的噬菌体T1和T4。分别测试了这些噬菌体在胰蛋白tone琼脂平板上的宿主细胞的体外裂解情况，其中大肠杆菌和噬菌体的浓度相等（1：1））（107 CFU和107 PFU），导致细菌菌落完全消失10分钟后。当噬菌体浓度降低时（1 :?和1 ::？），即使在10分钟后仍继续出现菌落。随后，将这些噬菌体混合并通过两周龄肉鸡的体内试验进行评估。将总共​​100只肉鸡分为四组（A，B，C和D），每组有25只鸡。前三组通过口服，腹膜内和肌内途径给予相同浓度（1：1）的噬菌体以及大肠杆菌（107 / ml）。对照组（D）进一步分为D1和D2两组，分别剥夺了噬菌体和大肠杆菌。 D2组的13只只给予噬菌体的禽类既没有显示疾病的迹象，也没有显示死亡率。结果表明，与对照组相比，使用噬菌体具有良好的保护水平。得出的结论是，噬菌体可以有效替代细菌已变得耐药的那些抗生素。

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