• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Istituto Superiore di Sanita. Rendiconti >Molecular characterization of Neisseria gonorrhoeae on non-cultured specimens from multiple anatomic sites
【24h】

Molecular characterization of Neisseria gonorrhoeae on non-cultured specimens from multiple anatomic sites

机译：淋病奈瑟菌在多个解剖部位非培养标本上的分子表征

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Introduction. The aim of this study was to molecularly characterize Neisseria gonorrhoeaeon non-cultured specimens collected from multiple anatomic sites. N. gonorrhoeaemultiantigen sequence typing (NG-MAST) together with the gene sequence analysis ofantimicrobial resistance (AMR) target genes were used.Materials and methods. Seventeen genital and extra-genital samples from eight patients(7 were men who have sex with men, MSM, and 1 women who have sex with men,WSM) with gonorrhoea symptoms were analyzed. For 7, of the 8 patients, conventionalculture method has been used to identify gonorrhoea. All the samples were tested withthe rapid molecular method CEPHEID. Amplification and sequencing of porB and tbpB,to identify the Sequence Type (ST) by NG-MAST, and penA, mtrR, porB1b, ponA geneswere also performed. Antimicrobial susceptibility by Etest, for the available culture positivesamples, was carried out.Results. For 7 patients the ST was obtained and for 6 the complete sequence analysisof the AMR target genes was also defined. For the majority of them, samples collectedfrom multiple sites (oropharynx, rectum, vaginal and urethra) confirm the presence ofthe same gonorrhoea strain. In particular, for 5 patients the same STs and changes in theAMR target genes were identified.Conclusion. Molecular characterization on non-cultured or culture negative specimensfor gonorrhoea can successfully be applied directly to genital and extra-genital samples.Thus permit to identify the presence of the same strain in patients with gonorrhoea infectionin multiple anatomic sites and to predict the antimicrobial susceptibility pattern.
机译：介绍。这项研究的目的是对从多个解剖部位收集的淋病奈瑟氏球菌非培养标本进行分子表征。使用淋病奈瑟球菌多抗原序列分型（NG-MAST）以及抗药性（AMR）目标基因的基因序列分析。材料和方法。分析了八名患者的十七个生殖器和生殖器外样本（7名男男性接触者，MSM，1名男男性接触者，WSM）的淋病症状。对于8例患者中的7例，已采用常规培养方法来鉴定淋病。所有样品均采用快速分子方法CEPHEID进行测试。 porB和tbpB的扩增和测序，以通过NG-MAST鉴定序列类型（ST），还进行了penA，mtrR，porB1b，ponA基因的研究。通过Etest对可用的培养阳性样品进行了药敏试验。结果。对于7例患者，获得了ST，对于6例，还定义了AMR靶基因的完整序列分析。对于大多数人来说，从多个部位（口咽，直肠，阴道和尿道）收集的样本证实存在相同的淋病菌株。特别是，对于5例患者，确定了相同的ST和AMR靶基因的变化。未培养或培养阴性的淋病标本的分子鉴定可以成功地直接应用于生殖器和生殖器外样品，从而可以在多个解剖部位鉴定淋病感染患者中相同菌株的存在并预测抗菌药敏感性模式。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号