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首页> 外文期刊>Iranian Journal of Microbiology >Antimicrobial effects of Ferula GummosaBoiss gum against extended-spectrum β-lactamase producing Acinetobacterclinical isolates
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Antimicrobial effects of Ferula GummosaBoiss gum against extended-spectrum β-lactamase producing Acinetobacterclinical isolates

机译：阿魏胶对产超广谱β-内酰胺酶的不动杆菌临床分离株的抗菌作用

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Background and Objectives: Acinetobacter spp. are important causes of nosocomial infections. They possess various antibiotic resistance mechanisms including extended spectrum beta lactamases (ESBLs). The aim of this study was to determine antibiotic resistance profile of Acinetobacter clinical isolates especially among ESBL-producing strains and to investigate the antimicrobial effects of oleo-gum-resin extract and essential oil of Ferula gummosa Boiss.Materials and Methods: 120 Acinetobacter strains were isolated from various clinical samples of hospitalized patients in Baqiyatallah hospital, Tehran during 2011-2012. Antibiotic susceptibility test was performed on the isolates using disk diffusion method. To detect and confirm the ESBL-positive isolates, phenotypic and genotypic tests were performed. Three types of F. gummosa oleo-gum-resin extracts and essential oils were prepared and the bioactive components of F. gummosa Boiss extracts were determined by GC-Mass chromatography. F. gummosa antimicrobial activity was evaluated against standard strain of Acinetobacter baumannii (ATCC19606) as well as Acinetobacter clinical isolates using well and disk diffusion methods. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth microdilution method.Results: 46 isolates were resistant to all tested antibiotics. All clinical isolates were resistant to cefotaxime. 12.94% of the isolates were phenotypically ESBL-producing among which 94.2% carried ESBL genes (blaPER-1, blaOXA-4 and blaCTX-M) detected by PCR. Oleo-gum-resin of F. gummosa had significant antibacterial activity and alcoholic essential oil had higher inhibitory effect on Acinetobacter strains (MIC of 18.75 mg/ml).Conclusion: Ferula gummosa extract contained components with well-known antimicrobial effects.
机译：背景与目的：不动杆菌属。是医院感染的重要原因。它们具有多种抗生素抗性机制，包括超广谱β-内酰胺酶（ESBLs）。这项研究的目的是确定不动杆菌临床分离株的抗药性，特别是在产ESBL的菌株中，并研究油胶树脂提取物和Ferula gummosa Boiss精油的抗菌作用。材料与方法：120株不动杆菌于2011-2012年从德黑兰Baqiyatallah医院住院患者的各种临床样本中分离得到。使用圆盘扩散法对分离物进行了抗生素敏感性试验。为了检测和确认ESBL阳性分离株，进行了表型和基因型测试。制备了三种类型的角果油树脂树脂提取物和香精油，并通过GC-Mass色谱法测定了角果提取物的生物活性成分。使用孔和圆盘扩散法评估鲍莫不动杆菌对标准菌株鲍曼不动杆菌（ATCC19606）以及不动杆菌临床分离株的抗菌活性。肉汤微量稀释法测定最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果：46株菌株对所有测试抗生素均具有抗性。所有临床分离株均对头孢噻肟耐药。表型上产生ESBL的菌株占12.94％，其中94.2％携带通过PCR检测到的ESBL基因（blaPER-1，blaOXA-4和blaCTX-M）。 F. gummosa的油脂树脂具有显着的抗菌活性，酒精精油对不动杆菌菌株的抑制作用更高（MIC为18.75 mg / ml）。结论：Ferula gummosa提取物中含有具有众所周知的抗菌作用的成分。

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