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首页> 外文期刊>Egyptian Journal of Medical Human Genetics >Salivary PCR detection of Helicobacter pylori DNA in Egyptian patients with dyspepsia
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Salivary PCR detection of Helicobacter pylori DNA in Egyptian patients with dyspepsia

机译：唾液PCR检测埃及消化不良患者的幽门螺杆菌 DNA

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Several methods are available for detecting Helicobacter pylori infection: (1) invasive methods based on gastric biopsies, (2) non invasive methods like Urea Breath Test (UBT), serology and stool antigen tests. Importance of salivary PCR in detection of H. pylori is still questionable. To evaluate the role of salivary PCR technique in detecting H. pylori gastric affection in Egyptian patients with dyspepsia and in differentiating between functional dyspepsia and acid-ulcer syndrome. This study included 60 patients with dyspepsia classified into three groups: (Group 1) patients with gastric H. pylori and ulcers or erosions ( n =20), (Group 2) patients with gastric H. pylori and no ulcers or erosions and had functional dyspepsia ( n =20), (Group 3) patients without H. pylori and had functional dyspepsia ( n =20). All underwent upper gastrointestinal endoscopy with biopsies, rapid urease test and salivary samples for H. pylori PCR. Significant difference between the three groups regarding salivary PCR values. No significant difference between Group 1 and Group 2 but both had significant difference with Group 3, significant difference between gastric H. pylori positive patients ( n =40) and negative ones ( n =20). Salivary PCR test had sensitivity of 85%, specificity of 70% in diagnosing H. pylori . PCR value of 534000Iu/ml had best sensitivity (75%) and specificity (100%) for diagnosing H. pylori , highly significant positive correlation between H. pylori gastric affection and salivary PCR values. No significant difference between patients with acid ulcer syndrome ( n =20) and those with functional dyspepsia ( n =40) as regard salivary PCR mean values. Salivary PCR test showed sensitivity of 100%, specificity of 50% in differentiating between patients with acid ulcer syndrome and those with functional dyspepsia. PCR value of 440000 Iu/ml had best sensitivity (100%) and specificity (55%) in differentiating acid ulcer syndrome from functional dyspepsia with non significant. H. pylori salivary PCR may be of value in diagnosing H. pylori gastric affection and is strongly correlated with it but it is of limited value in differentiating between acid ulcer syndrome and functional dyspepsia.
机译：有几种方法可用于检测幽门螺杆菌感染：（1）基于胃活检的侵入性方法，（2）非侵入性方法，例如尿素呼气试验（UBT），血清学和粪便抗原试验。唾液PCR在检测幽门螺杆菌中的重要性仍然值得怀疑。评估唾液PCR技术在埃及消化不良患者中检测幽门螺杆菌胃病以及区分功能性消化不良和酸溃疡综合征的作用。这项研究包括60位消化不良的患者，分为三组：（第1组）患有胃幽门螺杆菌并有溃疡或糜烂的患者（n = 20），（第2组）患有胃幽门螺旋杆菌且无溃疡或糜烂且具有功能的患者消化不良（n = 20），（第3组）无幽门螺杆菌且患有功能性消化不良（n = 20）。所有患者均接受上消化道内窥镜检查，并进行活检，快速尿素酶检测和唾液样本进行幽门螺杆菌PCR。唾液PCR值在三组之间有显着差异。第1组和第2组之间无显着性差异，但与第3组均具有显着性差异，胃幽门螺杆菌阳性患者（n = 40）和阴性患者（n = 20）之间有显着差异。唾液PCR检测在诊断幽门螺杆菌中的敏感性为85％，特异性为70％。 PCR值534000Iu / ml对幽门螺杆菌的诊断具有最高的敏感性（75％）和特异性（100％），幽门螺杆菌的胃病情与唾液PCR值之间呈极显着的正相关。唾液PCR平均值在酸性溃疡综合症患者（n = 20）和功能性消化不良患者（n = 40）之间无显着差异。唾液PCR试验显示，在区分酸性溃疡综合征和功能性消化不良的患者中，灵敏度为100％，特异性为50％。 PCR值440000 Iu / ml对区分酸性溃疡综合征和功能性消化不良的敏感性最高（100％），特异性最高（55％），无显着性。幽门螺杆菌唾液PCR可能在诊断幽门螺杆菌胃部疾病中具有重要价值，并且与幽门螺杆菌的胃部疾病有很强的相关性，但是在区分酸性溃疡综合征和功能性消化不良方面价值有限。

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