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Growth factor-induced osteogenesis in a novel radiolucent bone chamber

机译：生长因子诱导的新型射线可透性骨腔中的成骨作用

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Treatment of large bone defects is currently performed using mainly autograft or allograft bone. There are important drawbacks to bone grafting, such as limited availability, donor site morbidity in the case of autograft and inferior performance of allografts. Therefore, there is a great need for a suitable bone graft substitute. In order to evaluate efficiently newly developed biomaterials and factors intended for orthopaedic surgery, the bone chamber is a very suitable model. To allow longitudinal investigation of bone growth with μCT, a new bone chamber made of radiolucent polyether ether ketone (PEEK) was developed and studied for its feasibility. Therefore, PEEK bone chambers were placed on rat tibiae, and filled with vehicle (Matrigel without growth factors, negative controls), with bone morphogenetic protein 2 (BMP-2, positive controls), or a mix of growth factors combining BMP-2, vascular endothelial growth factor and the chemokine stromal cell-derived factor 1α, all laden on gelatin microspheres for controlled release (combined growth factors). Growth factor presence led to a significant increase in bone formation after 8 weeks, which subsided after 12 weeks, underlining the importance of longitudinal analysis. We conclude that the PEEK-bone chamber is a suitable translational animal model to assess orthotopic bone formation in a longitudinal manner.
机译：目前主要使用自体移植或同种异体移植骨进行大骨缺损的治疗。骨移植存在重要的缺点，例如可用性有限，自体移植时供体部位发病率高以及同种异体移植物的性能较差。因此，非常需要合适的骨移植​​物替代物。为了有效地评估用于骨科手术的新开发的生物材料和因素，骨腔是非常合适的模型。为了能够用μCT对骨生长进行纵向研究，开发了一种由射线可透性聚醚醚酮（PEEK）制成的新骨腔，并对其可行性进行了研究。因此，将PEEK骨腔放置在大鼠胫骨上，并充满媒介物（不含生长因子的Matrigel，阴性对照），骨形态发生蛋白2（BMP-2，阳性对照）或结合BMP-2的生长因子混合物，血管内皮生长因子和趋化因子基质细胞衍生因子1α均载于明胶微球上以进行控制释放（联合生长因子）。生长因子的存在导致8周后骨形成的显着增加，而12周后逐渐消失，这突显了纵向分析的重要性。我们得出结论，PEEK骨腔是一种合适的转化动物模型，可以纵向评估原位骨的形成。

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